【摘要】 目的 探索建立广西巴马小型猪阴道黏膜免疫炎症反应模型的方法。方法 将6只雌性小型猪随机分为基质对照组和壬苯醇醚-9(N-9)模型组(40 mg/mL)(每组3只)。肌肉注射苯甲酸雌二醇12 d后，各组阴道给药5 mL/d，连续7 d。造模后取阴道黏膜组织进行病理检查，并根据上皮溃疡、坏死、细胞浸润、出血、充血、水肿等指标进行病理评分;造模后24 h与72 h，采集阴道灌流液(CVL),采用流式细胞术对CVL沉淀细胞中的中性粒细胞比例和CD45+细胞百分率进行分析;免疫组织化学法(IHC)检测阴道黏膜组织中CD45+细胞的表达及分布。结果 组织病理学观察显示，N-9模型组阴道黏膜上皮损伤明显，出现不同程度的溃疡、坏死、炎性浸润、出血、水肿等。病理评分结果显示，N-9模型组病理总分值(18.0±3.2)与基质对照组(3.3±0.2)相比明显升高(P<0.01)。N-9模型组CVL的中性粒细胞比例为41.70%±1.63%(24 h)和51.17%±3.81%(72 h), CD45+细胞比例为58.5%±4.73%(24 h)和88.0%±3.57%(72 h)，与基质对照组相比均显著增高(P<0.01)。N-9模型组阴道黏膜CD45+细胞表达明显增多，累积光密度值为33.95±6.69，显著高于基质对照组(23.42±3.30)(P<0.05)。结论 阴道连续给予N-9凝胶7 d可以诱发小型猪阴道黏膜明显的炎性反应，符合阴道黏膜免疫炎症反应模型的特征。更多还原

【Abstract】 Objective To establish the vaginal mucosal immuno-inflammatory model response of minipig.Methods Six female minipigs were randomly divided into the control and Nonoxynol-9 (N-9)model group (40 mg/mL).After 12 days of estradiol benzoate intramuscular injection,the test drugs (N-9)were administered through vagina to minipigs at 5 mL/d for 7 consecutive days.On the 7th day,histopathological examination of vaginal mucosa was undertaken and the pathological scores were calculated based on histological indexes including epithelial ulceration/erosion,necrosis,cell infiltration,hemorrhage,congestion and edema.Cervicovaginal lavage (CVL) was collected at 24 h and 72 h after drug administration.The percentage of neutrophilic granulocytes and CD45~+cells in the CVL deposited cells were detected by flow cytometry.The expression and location of CD45~+cells in the vaginal mucosa were assessed by using immuno-histochemical (IHC) assay.Results Histopathological examination showed obvious lesions including inflammation,bleeding and edema in the vaginal epithelium of the N-9 group.Histological score of the N-9 group (18.0±3.2) was significantly higher than that of the control (3.3±0.2)(P<0.01).The percentage of neutrophilic granulocytes in the CVL cells of N-9 group was (41.70±1.63)%(24 h) and (51.17±3.81)%(72 h),and the percentage of CD45~+cells in the CVL cells of N-9 group was(58.5±4.73)%(24 h) and (88.0±3.57)%(72 h),both showed significant increase compared to that of the control (P<0.01).CD45~+cell expression increased significantly in the N-9 group.The IOD value of N-9group was 33.95±6.69,which was significantly higher than that of the control group (28.42±8.96)(P<0.05).Conslusion The vaginal mucosal immuno-inflammatory response model of mini-pigs could be induced by giving 40 mg/m L N-9 gel to the mini-pigs through vagina for 7 consecutive days.更多还原