Combinatorial analyses including enzymatic analysis and atomic force microscopy(AFM) were used to gain more insights into structure of the alkali-denatured plasmid p BR322 DNA(DNA Ⅳ).Among the restriction enzymes tested,PstⅠ was able to cleave the p BR322 DNA Ⅳ molecules,indicative of the existence of an intact restriction site in the denatured DNA.AFM images revealed that PstⅠ-treated DNA Ⅳ molecules contained an individual node.The contour length of the condensed molecules was decreased by about 11% compa...
【基金】
supported by grants from The National Natural Science Foundation of China(31270124,30900023)~~
【更新日期】
2016-08-19
【分类号】
Q523
【正文快照】
DNA is the most important genetic material of thecellular organisms.The structure of DNA has greateffects on its functions in cells.A double-strandedDNA molecule has been demonstrated to be one of thethree structural forms:supercoiled DNA(DNAⅠ),relaxed o
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