【摘要】 以长粒品种‘三粒寸’‘IAC1246’‘JAPPENI TUNGKUNGO’和‘MIGA’作为供体亲本,以优良选系SAGC-4作为受体亲本,进行杂交和连续回交,利用GS3基因的功能性分子标记SF28进行BC₁F₁和BC₂F₁的分子标记辅助选择。在不同BC₂F₁单株派生的BC₂F₂分离群体中,观察到粒长性状的典型双峰分布或单峰连续分布。结合田间农艺性状考察,选出89个具备细长粒型且综合农艺性状良好的单株,其中4个优良单株的平均粒长从原始受体亲本的8.32 mm增加至9.84 mm左右,平均长宽比由原来的2.61增加至3.00。结果表明:GS3基因具有控制粒长和长宽比的较大遗传效应,对该基因进行分子标记辅助选择可快速改良亲本的粒型性状。更多还原

【Abstract】 Four donor parental lines with long grain shape,’ SANLICUN’,’ IAC1246 ’,’ JAPPENI TUNGKUNGO’ and ’ MIGA’,were crossed with a promising indica rice line SAGC-4 as the recipient parent,followed by continuous backcrosses.Marker-assisted selection was conducted in both BC₁F₁ and BC₂F₁ generations by using the CAPs marker SF28 of GS3 gene.Segregation of grain length showed typical bimodal or continuous distributions in BC₂F₂ populations.A set of 89 individuals were selected based on both grain shape and visual inspection on other agronomic traits.Among them,4 promising individuals had average grain length of 9.84 mm and length/width ratio of 3.00,in comparison to 8.32 mm and 2.61 of the original recipient parent,respectively.The results indicated that GS3 gene had large genetic effects on both grain length and length/width ratio.Markerassisted selection of the gene could accelerate the improvement of rice grain shape.更多还原