【摘要】 目的 :研究p53上调凋亡调控因子(p53 up-regulated modulator of apoptosis,PUMA)在唾液腺腺样囊性癌(salivary cystic carcinoma,SACC)癌组织及癌旁组织中的表达,探讨PUMA在SACC发生、发展中的作用。方法 :利用实时定量PCR(RT-PCR)和Western免疫印迹法分别检测27例SACC癌旁组织和27例SACC癌组织中PUMA基因和PUMA蛋白的表达。采用SPSS19.0软件包对数据进行统计学分析。结果:以SACC癌旁组织作为对照样本,其PUMA基因表达相对定量为1,SACC癌组织中PUMA基因表达相对定量为0.57±0.17。PUMA蛋白在SACC癌旁组织和癌组织中表达的相对量分别为0.94±0.12和0.36±0.12。SACC癌组织中PUMA基因和PUMA蛋白的表达显著低于SACC癌旁组织(P<0.05)。结论:PUMA基因在SACC中的表达下调,与SACC的发生呈负相关。更多还原

【Abstract】 PURPOSE: To explore the expression of p53 up-regulated modulator of apoptosis(PUMA) gene in salivary adenoid cystic carcinoma(SACC) and its clinical significance. METHODS: The expressions of PUMA gene and protein were detected in 27 SACC tumor-adjacent tissues(group A) and 27 SACC tumor tissues(group B) with real-time quantitative PCR and Western blot. Statistical analysis was performed using SPSS 19.0 software package. RESULTS: The expression of PUMA gene in groups A as calibrator was 1,and expression of PUMA gene in group B was 0.57 ±0.17. The expression of PUMA protein in groups A and B were 0.94 ±0.12 and 0.36 ±0.12,respectively. The expressions of PUMA gene and protein were significantly lower in group B than in group A(P<0.05). CONCLUSIONS: The expression of puma gene in SACC tissues is down-regulated, which is negatively correlated with the development of SACC.更多还原