【摘要】 目的探讨沉默信息调节因子1(SIRT1)在结直肠癌组织中的表达及其与多药耐药相关蛋白1(MDR-1)的关系。方法选择结直肠癌患者162例,取手术切除的癌组织,采用免疫组化Envision法观察SIRT1、MDR-1的阳性表达,分析结直肠癌组织SIRT1阳性表达与患者临床病理参数及MDR-1阳性表达的关系。结果结直肠癌组织中SIRT1、MDR-1阳性表达率分别为66.0%(107/162)和59.3%(96/162)。结直肠癌组织SIRT1阳性表达与患者性别、肿瘤组织分化程度、临床分期及淋巴结转移无关(P均>0.05),与肿瘤长径有关(P<0.05)。结直肠癌组织SIRT1阳性表达与MDR-1阳性表达呈正相关(r=0.506,P<0.01)。结论结直肠癌组织中SIRT1阳性表达较高,其阳性表达与MDR-1阳性表达呈正相关关系。更多还原

【Abstract】 Objective To investigate the expression of silent information regulator 1( SIRT1) in colorectal carcinoma( CRC) and its relationship with and multidrug resistance-related protein 1( MDR-1). Methods We used the imunohistochemistry method to detect the expression of SIRT1 and MDR-1 and analyzed their relationships with clinical data in 162 specimens of CRC. Results SIRT1 and MDR-1 expression was detected in 107( 66. 0%) and 96( 59. 2%) out of 162 colorectal adenocarcinomas,respectively. Statistically,the expression of the SIRT1 protein was not associated with clinical and pathological parameters,including sex,tumor stage,lymph node metastasis and tumor differentiation( all P > 0. 05),but was associated with tumor size( P < 0. 05). In addition,the positive expression of SIRT1 and MDR-1 in CRC was positively correlated with each other( r = 0. 506,P < 0. 01). Conclusion SIRT1 is highly expressed in the CRC and its expression is positively correlated with MDR-1 expression.更多还原