【摘要】 目的观察法舒地尔联合罗格列酮对脂多糖(LPS)诱导的人冠状动脉(冠脉)内皮细胞(HCAEC)血管细胞黏附因子1(VCAM-1)、单核细胞趋化因子1(MCP-1)表达的影响。方法选用体外培养的第3~5代HECAC进行实验,将细胞分为空白对照组、LPS组(100 ng/m L LPS作用细胞)、法舒地尔组(10 nmol/L法舒地尔作用细胞)、罗格列酮组(10μg/m L罗格列酮作用细胞)、LPS+法舒地尔组(先予10 nmol/L的法舒地尔干预细胞2 h后再给予100 ng/m L LPS共同干预22 h)、LPS+法舒地尔+罗格列酮组(先予10 nmol/L的法舒地尔和10μg/m L罗格列酮干预细胞2 h后再给予浓度为100 ng/m L LPS共同干预22 h),实时荧光定量PCR检测各组细胞VCAM-1、MCP-1mRNA,ELISA法检测细胞上清液中可溶性VCAM-1、MCP-1蛋白。结果与空白对照组相比,LPS组细胞VCAM-1、MCP-1 mRNA及上清液VCAM-1、MCP-1蛋白表达高(P均<0.05)。与LPS组相比,LPS+法舒地尔组细胞VCAM-1、MCP-1 mRNA及上清液VCAM-1、MCP-1蛋白表达低(P均<0.05)。与LPS+法舒地尔组相比,LPS+法舒地尔+罗格列酮组细胞VCAM-1、MCP-1 mRNA及上清液VCAM-1、MCP-1蛋白表达低(P均<0.05)。结论法舒地尔联合罗格列酮可协同抑制LPS诱导的HCAEC中VCAM-1、MCP-1的表达,可能与两者共同抑制p38MAPK、NF-κB信号通路有关。更多还原

【Abstract】 Objective To observe the effects of fasudil combined with rosiglitazone on the expression of vascular cell adhesion molecule-1(VCAM-1) and monocyte chemotactic protein 1(MCP-1) in lipopolysaccharide(LPS)-induced human coronary arterial endothelial cells(HCAECs).Methods We conducted the experiment using the 3rd to the 5th generation of HCAECs in vitro.Then,the cells were divided into the control group(non-intervention),LPS group(HCAECs were intervened with 100 ng/m L LPS),fasudil group(HCAECs were intervened with 10 nmol/L fasudil),rosiglitazone group(HCAECs were intervened with 10 μg/m L rosiglitazone),LPS + fasudil group(HCAECs were intervened with 10 nmol/L fasudil for 2 h followed by 100 ng/m L LPS for 22 h),LPS + fasudil + rosiglitazone group(HCAECs were intervened with 10 nmol/L fasudil and 10 μg/m L rosiglitazone for 2 h followed with 100 ng/m L LPS together for 22 h).The protein expression of VCAM-1 and MCP-1 in supernatant was detected by ELISA and the mRNA was detected by real-time fluorescent quantification PCR.Results Compared with the control group,the mRNA expression of VCAM-1 and MCP-1,and the protein expression of VCAM-1 and MCP-1 was increased in the LPS group(all P < 0.05).Compared with the LPS group,the mRNA expression of VCAM-1 and MCP-1,and the protein expression of VCAM-1 and MCP-1 was decreased in the LPS + fasudil group(all P < 0.05).Compared with LPS + fasudil group,the mRNA expression of VCAM-1 and MCP-1,and the protein expression of VCAM-1 and MCP-1 was decreased in the LPS + fasudil + rosiglitazone group(all P <0.05).Conclusion Fasudil combined with rosiglitazone collaboratively inhibits the expression of VCAM-1 and MCP-1 in HCAECs induced by LPS,which may be related to their co-suppression of p38 MAPK and NF-κB signaling pathway.更多还原