【摘要】 目的观察川芎嗪腹腔注射治疗大鼠偏头痛的效果,并探讨其可能机制。方法雄性SD大鼠30只,随机分为3组各10只,A组大鼠制模前腹腔注射盐酸川芎嗪注射液,B、C组腹腔注射等量生理盐水,连续7 d。A、B组末次给药30 min后左肩部皮下注射硝酸甘油注射剂以制备偏头痛模型,C组同期左肩部皮下注射生理盐水0.5m L。观察各组造模后各时间段爬笼、挠头次数,采用免疫荧光法、Western blotting法检测各组三叉神经颈髓复合体(TCC)中瞬时受体电位香草酸亚型1(TRPV1)蛋白,实时荧光定量PCR技术检测TRPV1 mRNA。结果与B组比较,A、C组制模后各时段大鼠爬笼次数、挠头次数均减少(除A、B组制模后0~30 min大鼠挠头次数比较外,P均<0.05)。各组大鼠TCC内均有TRPV1不同程度阳性表达,B组表达亮度较A、C组明显。与B组比较,A、C组的TRPV1蛋白、mRNA相对表达量减少(P均<0.01)。结论川芎嗪腹腔注射对偏头痛大鼠具有一定的治疗作用,其作用机制可能与抑制TCC中TRPV1表达有关。更多还原

【Abstract】 Objective To explore the effect and mechanism of intraperitoneal injection of tetramethylpyrazine for the treatment of migraine in rats. Methods Thirty Sprague-Dawle( SD) rats were randomly divided into three groups of 10 in each group: groups A,B and C. Rats in the group A were given intraperitoneal injection of ligustrazine hydrochloride injection before modeling,groups B and C were given intraperitoneal injection of the same volume of normal saline for 7 days. Migraine rat model was established by subcutaneously injection of nitroglycerin after 30 min of the last delivery in the groups A and B,and group C with subcutaneous injection of 0. 5 m L normal saline. We observed the frequency of scratching head and climbing cage of each rat. The expression of transient receptor potential cation channel subfamily V member 1( TRPV1) protein in rat trigeminocervical complex( TCC) was detected with Western blotting,and the expression of TRPV1 mRNA was detected by real-time fluorescence quantificative PCR. Results Compared with group B,the frequency of scratching head and climbing cage in each period was significantly decreased in the groups A and C( except the frequency of scratching head between groups A and B in the 0-30 min after molding,statistical difference was found,all P < 0. 05). Different levels of positive expression of TRPV1 receptors in TCC of each group were observed by immunofluorescence. Compared with groups A and C,the expression intensity was higher in the group B,there was no statistical difference between group A and B. Compared with group B,the protein and mRNA expression of TRPV1 in TCC of group A and C were significantly decreased( all P <0. 01). Conclusion Intraperitoneal injection of tetramethylpyrazine on migraine rats has a certain therapeutic effect,and the mechanism may be related to the inhibition of TRPV1 expression in trigeminal sensory neurons.更多还原