【摘要】 目的观察人参皂苷Rg3对人结肠癌细胞株SW480增殖的影响,并探讨其可能作用机制。方法取对数生长期SW480细胞,分别加入含160、80、40、0μmol/L人参皂苷Rg3的储备液(分别计为A、B、C、D组)培养24 h,采用MTT法检测人参皂苷Rg3对SW480细胞增殖活性(OD值)的影响,倒置显微镜观察人参皂苷Rg3诱导SW480细胞凋亡形态学改变,流式细胞术检测SW480细胞凋亡率,RT-PCR法检测细胞间黏附分子1(ICAM-1)、闭锁蛋白(Occludin)mRNA,Western blotting法检测ICAM-1、Occludin蛋白。结果与D组比较,A、B、C组细胞OD值下降,凋亡率增加,ICAM-1 mRNA、蛋白表达降低,Occludin mRNA、蛋白表达升高(P均<0.05)。结论人参皂苷Rg3可抑制SW480细胞增殖,促进其凋亡,机制可能与ICAM-1表达下调、Occludin表达上调有关。更多还原

【Abstract】 Objective To observe the effect of ginsenoside Rg3 on the proliferation of human colon cancer cell line SW480,and to investigate its mechanism. Methods SW480 cells in the logarithmic phase were exposed to 160,80,40 and 0 μmol / L ginsenoside Rg3 for 24 hours( marked as groups A,B,C and D). MTT assay was used to measure the effect of ginsenoside Rg3 on the cell proliferative activity( OD value). Invert microscope was used to observe the morphological changes of SW480 cells,and flow cytometry was employed to measure the apoptosis of SW480 cells. The intercellular adhesion molecule 1( ICAM-1) and occludin mRNA was measured by reverse transcription polymerase chain reaction( RT-PCR),and Western blotting was used to detect the expression of ICAM-1 and occludin protein. Results The OD values were decreased,the apoptosis rates were increased,the expression of ICAM-1 mRNA and protein was decreased,and the expression of occludin mRNA and protein was increased in the groups A,B and C as compared with those of group D( all P < 0. 05). Conclusion Ginsenoside Rg3 can inhibit the proliferation of SW480 cells and induce apoptosis,and its mechanism may be related with the down-regulated expression of ICAM-1 and up-regulated expression of occludin.更多还原