【摘要】 目的分析2015年1月~2015年12月我院血流感染患者主要病原菌的构成及耐药性,为临床血流感染的诊断与治疗提供依据。方法血液培养采用美国BD公司Bactec FX型和法国梅里埃公司Bact/Alert 3D 240型血培养仪;细菌鉴定采用德国Walkway 96 Plus和法国梅里埃API鉴定系统;抗菌药物敏感试验分别采用Micro Scan药敏板联合K-B纸片扩散法;药物敏感性结果统计分析采用Whonet 5.6软件。结果血流感染患者中革兰阳性菌检出率为34.55%,革兰阴性菌检出率为63.11%,其中革兰阴性杆菌主要为大肠埃希菌(31.69%)和肺炎克雷伯菌(8.83%),革兰阳性菌主要为表皮葡萄球菌(8.05%)和金黄色葡萄球菌(7.53%);葡萄球菌、肠球菌和肺炎链球菌对万古霉素、利奈唑胺100%敏感,对红霉素和克林霉素耐药率均高于70%;大肠埃希菌、肺炎克雷伯菌、弗氏枸橼酸杆菌和阴沟肠杆菌对亚胺培南和美罗培南100%敏感,铜绿假单胞菌对亚胺培南和美罗培南耐药率均为15.4%,大肠埃希菌和肺炎克雷伯菌对头孢曲松的耐药率分别47.5%和32.4%,对左氧氟沙星的耐药率分别51.6%和20.6%。结论我院血流感染病原菌以革兰阴性菌为主,大肠埃希菌和凝固酶阴性葡萄球菌耐药严重,临床应重视血培养标本送检和细菌耐药监测,合理使用抗菌药物。更多还原

【Abstract】 Objective To get information about bacterial distribution and resistance in bloodstream infection.Methods Various clinical department blood cultures samples were collected by Bactec FX and Bact / Alert 3D 240.The identification and antimicrobial susceptibility testing were performed with Walkway 96 Plus and API,Statistical analysis was done with Whonet 5.6.Results A total of 385 bacterial strains were isolated,and gram negative bacteria accounted for 63.11%,of which Escherichia coli accounted for 31.69%,followed by Klebsiella pneumonia 8.83%,the gram positive bacteria accounting for 34. 55%,of which Staphylococcus epidermidis accounted for 8. 05%,followed by staphylococcus aureus accounted for7. 53%.The resistant rates of Staphylococcus,Enterococcus and Streptococcus pneumoniae to vancomycin,linezolid were 0; No imipenem and meropenem resistant Escherichia coli and Klebsiella pneumonia were found. Conclusion Gram-negative bacilli were the major pathogens of BSI; Antibiotic resistance becomes more prevalent in some major pathogens.Rational use of antibiotics is important for the containment of bacterial resistance.更多还原