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生物酶对五氯联苯降解的初步研究

Pilot study on bioenzyme degradation to pentachlorodiphenyl

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【作者】 季蕾陈贯虹傅晓文黄玉杰王加宁张强

【Author】 JI Lei;CHEN Guan-hong;FU Xiao-wen;HUAGN Yu-jie;WANG Jia-ning;ZHANG Qiang;Shandong Provincial Key Laboratory of Applied Microbiology,Ecology Institute,Shandong Academy of Sciences;

【机构】 山东省科学院生态研究所山东省应用微生物重点实验室

【摘要】 多氯联苯(PCBs)是具有代表性的持久性有机污染物(POPs),其氯代数目越多,降解越难。本文以分离筛选的6株PCBs降解细菌为材料,制备了含有NADH辅酶再生系统关键酶甲酸脱氢酶(FDH)的PCBs降解复合酶,研究了其对PCBs的降解效果。结果表明,Pseudomonas sp.MGB11胞内酶/FDH复合酶对五氯联苯PCB114的降解率在10 h内可达69.4%,对于高毒性、难降解的高氯PCBs的污染修复具有应用价值。

【Abstract】 Polychlorinated biphenyls( PCBs) is a representative of persistent organic pollutants( POPs). More its chlorinated number leads to more difficult its degradation. We prepared PCBs compound degrading enzyme of key enzyme formate dehydrogenase( FDH) of NADH coenzyme regeneration system with isolated 6 PCBs degradation bacteria strains as materials. We address its degrading effect to PCBs. Results indicate that degradation rate of Pseudomonas sp. MGB11/FDH compound enzyme to pentachlorodiphenyl PCB114 is up to 69. 4% within 10 h. It therefore has application value in the repair for high chlorine PCBs pollutants with high toxicity and low degradability.

【基金】 国家国际科技合作专项(2014DFE90100);山东省自然科学基金(ZR2015YL008,BS2015HZ011)
  • 【分类号】X592;X172
  • 【被引频次】1
  • 【下载频次】97
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