To explore the influence about the expression levels of the two enzymes( SHAN and Lux S) in E. coli cells and quorum sensing of bacteria,the gene sahn and lux S were amplified by PCR,and then linked to vector p ET- 28 a so as to construct p ET- 28a-SAHN and p ET- 28a- Lux S,which were transformed into E. coli BL21. Expressions of SAHN and Lux S were induced by IPTG. The two enzymes were excessive expressed in the recombinant strains,so they promoted the biochemical pathways about quorum sensing of bacteria....
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