【摘要】 目的:研究化积益肝煎对小鼠肝癌腹水瘤细胞系H22治疗的作用机制。方法:小鼠右腋下皮下种瘤造成实体瘤模型,将24只小鼠分为三组,生理盐水组、化积益肝煎组(中药组)和5-氟脲嘧啶组(西药组)各8只,用药14天后取瘤,称重计算抑瘤率,采用免疫组化技术检测C–erb B-2及Fasl的表达。结果:中药组、西药组大鼠瘤重显著低于对照组(P<0.05);中药组、西药组的抑瘤率分别为32.11%、38.61%;C–erb B-2及Fasl在三组中表达结果经秩和检验,与对照组比较,中药组C–erb B-2、Fasl表达减弱,差异均有统计学意义(P<0.05),而与西药组比较差异无统计学意义(P>0.05)。结论:化积益肝煎对肝癌H22细胞的增殖有明显的抑制作用,其作用机制与C–erb B-2、Fasl表达有关。更多还原

【Abstract】 Objective: To study the mechanism of using Huaji Yigan Decoction for treating ascites hepatoma cell line H22.Methods: After injecting ascites hepatoma cell line H22 ceils into their right oxter,24 mice were divided into three groups: the controlled group received treatment of normal solution,the treatment group treated by Huaji Yigan Decoction and the treatment group treated by 5-Fu.After 14 days inhibiting rate was accounted,C-erbB-2and Fasl were examined by using immunohistochemistry straining method in the tumor tissue.Results: The inhibiting rate was 32.11% in hepatoma cell by using Huaji Yigan Decoction.C-erbB-2and Fasl in the three groups were positive.One-factor analysis of variance manifest: the expression of C-erbB-2and Fasl was decreased in the treatment group.The difference between the two groups were significant(P<0.05).The comparison between Huaji Yigan Decoction group and 5-Fu(P>0.05),were not statistically significant.Conclusion: H22 cellsbearing mice are treated with Huaji Yigan Decoction can significantly inhibit H22 cells,and the mechanism may is relative to expressions of C-erbB-2and Fasl.更多还原