Walnut anthracnose disease is a kind of serious disease of walnut all over the world. In this paper, the identification and the biological characteristics of strain TS-09 isolated from the walnut fruits were studied. The results showed that the strain TS-09 was identified as Colletotrichum gloeosporioides based on its morphologic characters, cultural characteristics and four conserved genes(ribosomal DNA-ITS, ACT, β-tub2 and GPDH)sequences analysis and the pathogenicity was powerful. For the growth of strai...