【摘要】 本研究主要探索油菜中E3泛素连接酶BnTR1在拟南芥中的同源基因AtTR1(At3g47550)的功能.通过体外泛素化实验证明AtTR1具有E3连接酶活性.基因表达分析显示该基因受200mmol/L NaCl显著诱导,说明该基因可能在响应盐胁迫中发挥一定的功能.为了更深入的探究该基因在植物耐盐中的作用,构建了植物表达载体pZH01-AtTR1转化突变体.在含有潮霉素的培养基上筛选阳性苗,并利用荧光定量PCR检测表明AtTR1基因已经成功转入突变体中.更多还原

【Abstract】 In this work,the function of AtTR1(At3g47550),the homologues of E3 ligase BnTR1in Arabidopsis was studied.In vitro self-ubiquitination assay demonstrated that the protein possess an E3 ligase activity.The analysis of expression pattern revealed that AtTR1 was dominantly induced by salt(200mmol NaCl).The result indicated that the gene may play a certain function in response to salt stress.In order to further study the function of the gene in plant salt resistance,the plant expression Vector pZH01-AtTR1 was constructed and introduced into mutants.Positive transgenic seedlings were selected for their ability to grow on a medium containing hygromycin.RT-PCR analysis of hygromycin resistant plants showed that AtTR1 gene had been integrated into the genome of attr1 mutant.更多还原