【摘要】 目的研究乳腺癌局部微环境中免疫相关细胞因子干扰素(IFN)-γ、白介素(IL)-10和转化生长因子(TGF)-βmRNA表达的状况,并与临床病理特征相比较,探讨其意义。方法 48例乳腺浸润性导管癌手术切除标本,根据是否淋巴结转移分为两组,采用地高辛末端标记的寡核苷酸探针,以原位杂交技术检测肿瘤浸润单个核细胞和肿瘤细胞IFN-γ、IL-10和TGF-βmRNA的表达状况。结果淋巴结转移组乳腺癌细胞TGF-β和IL-10 mRNA表达明显高于无淋巴结转移组(TGF-β[%/积分指数]:46.25±12.88/0.49±0.27 vs 19.33±7.69/0.28±0.18;IL-10[%/积分指数]:39.08±17.49/0.42±0.19 vs 22.58±7.87/0.33±0.17,t=4.330,P<0.01;t=2.962,P<0.05),TGF-βmRNA在乳腺癌细胞中表达的差别更为明显。未检出IFN-γmRNA阳性肿瘤细胞。肿瘤间质浸润的单个核细胞中,淋巴结转移组IFN-γmRNA阳性细胞明显低于TGF-β、IL-10 mRNA阳性细胞(TGF-β:35.58±13.62/0.40±0.25,IL-10:40.83±15.49/0.47±0.22,IFN-γ:18.17±8.41/0.18±0.08,t=3.005,P<0.05;t=4.390,P<0.01;t=3.563,P<0.01)。结论乳腺癌组织局部微环境中,调节T细胞和Th2免疫相关细胞因子mRNA表达明显占优势,使得肿瘤免疫微环境趋于抗瘤免疫反应抑制状态。更多还原

【Abstract】 Objective To investigate the expression status of immune related cytokines IFN-γ,IL-10 and TGF-β mRNA in breast cancerous microenvironment and make comparison with clinicopathologic features to explore its significance. Methods Forty-eight samples of breast cancer with excised axillary lymph nodes were included. The samples were divided into two groups according to lymph node metastatic status. Digoxin-labeled oligonucleotide probes and in situ hybridization technique were used to detect the expressions of IFN-γ,IL-10 and TGF-β mRNA in breast cancer cells and the tumor infiltrating mononuclear cells. Results The expressions of TGF-β and IL-10 mRNA in breast cancerous cells were significantly higher in node metastatic group compared to those of non-metastatic group( TGF-β[% /accumulative index]: 46. 25 ± 12. 88 /0. 49 ± 0. 27 vs 19. 33 ± 7. 69 /0. 28 ± 0. 18; IL-10[% / accumulative index]: 39. 08 ± 17. 49 /0. 42 ± 0. 19 vs 22. 58 ± 7. 87 /0. 33 ± 0. 17,t = 4. 330,P < 0. 01; t = 2. 962,P < 0. 05). The difference in expression of TGF-β mRNA in breast cancer cells was more significant. IFN-γ mRNA positive cancer cell was not detected. In tumor infiltrating mononuclear cells,the IFN-γ mRNA positive cells in node metastatic group were significantly less than TGF-β and IL-10 mRNA positive cell( TGF-β: 35. 58 ±13. 62 /0. 40 ± 0. 25,IL-10: 40. 83 ± 15. 49 /0. 47 ± 0. 22,IFN-γ: 18. 17 ± 8. 41 /0. 18 ± 0. 08,t = 3. 005,P < 0. 05; t = 4. 390,P < 0. 01;t = 3. 563,P < 0. 01). Conclusion Treg and Th2 related cytokines are predominated in local microenvironment of breast cancer and the microenvironments of breast cancer demonstrate an immunoinhibitory status in terms of anticancer immune response.更多还原