【摘要】 为研究G蛋白偶联受体87(GPR87)在氨基酸调控泌乳过程中的作用,在奶牛乳腺上皮细胞中分别添加浓度为0.6mmol/L蛋氨酸(Met)和1.2 mmol/L赖氨酸(Lys),检测细胞泌乳能力变化及细胞中GPR87的表达。结果表明,1添加Met和Lys均能使细胞泌乳能力和细胞中GPR87的表达显著增加;对GPR87进行过表达和抑制,检测细胞泌乳能力变化;GPR87过表达后,细胞泌乳能力显著增加,抑制后,细胞泌乳能力显著下降;2抑制GPR87同时添加Met或Lys,检测细胞中CSN2、m TOR、SREBP-1c、GLUT1的表达及细胞泌乳能力的变化;3与只添加氨基酸组比较,抑制GPR87同时添加氨基酸组,细胞中CSN2、m TOR、SREBP-1c、GLUT1的表达及细胞泌乳能力的增加程度显著下降。综合结果说明,GPR87能接受并传递氨基酸信号,正向调控奶牛乳腺上皮细胞的泌乳。更多还原

【Abstract】 The aim of this study was to investigate the effect of G protein coupled receptors 87(GPR87) on the process of amino acid-mediated regulation of lactation in dairy cow mammary epithelial cells(DCMECs). The DCMECs were treated with 0.6 mmol/L methionine(Met) and 1.2 mmol/L lysine(Lys), respectively, the lactation and GPR87 expression of these cells were detected, the result showed that lactation and GPR87 expression of DCMECs were significant increased by Met or Lys stimulus; GPR87 was overexpressed and inhibited in DCMECs, respectively, the lactation of these cells were detected, the result showed that lactation of DCMECs was significant increased by GPR87 overexpression and significant reduced by GPR87 inhibiton; Treated with GPR87 inhibition and Met or Lys stimulus, the lactation and the expression of CSN2,m TOR,SREBP-1c,GLUT1 of DCMECs were detected, the result showed that compared with only treated with Met or Lys stimulus group, the increase of lactation and the expression of CSN2,m TOR,SREBP-1c,GLUT1 of DCMECs treated with GPR87 inhibition and Met or Lys stimulus was significant reduced. Taken together, these results reveal that GPR87 as a regulation molecular could receive and pass the amino acid signal, and positive control the lactation of DCMECs.更多还原