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多杀菌素·毒死蜱增效混配及其对AchE和CarE的联合作用

Larvicidal Interaction of the Mixture of Spinosad and Chlorpyrifos with a Synergistic Effect on Acetylcholinesterase and Carboxylesterase

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【作者】 杨超; 沈雪晴; 张世玮; 肖慈英; 黄青春;

【Author】 YANG Chao;SHEN Xue-qing;ZHANG Shi-wei;XIAO Ci-ying;HUANG Qing-chun;Shanghai Key Lab of Chemical Biology,School of Pharmacy,East China University of Science and Technology;

【机构】 华东理工大学药学院上海市化学生物学重点实验室;

【摘要】 [目的]研究多杀菌素(SPY)和毒死蜱(CHP)复配剂对3龄黏虫(Mythimna separata)的室内毒力及其对乙酰胆碱酯酶(ACh E)和羧酸酯酶(Car E)的联合作用。[方法]采用交互测定法。[结果]SPY∶CHP(1∶9)中单剂毒力具有相加作用,对ACh E和Car E的抑制作用显著高于毒死蜱;而SPY∶CHP(9∶1)中单剂毒力具有显著的增效作用,对ACh E抑制作用与毒死蜱无显著差异,对Car E的抑制作用仍显著高于毒死蜱。[结论]多杀菌素对毒死蜱的ACh E和Car E抑制作用具有诱导效应。

【Abstract】 [Aims] This study aims to investigate the synergistic interaction of the mixture of spinosyns(SPY) and chlorpyrifos(CHP) against Mythimna separata larvae and its combined effect on acetylcholinesterase(ACh E) and carboxylesterase(Car E). [Methods] Indoor toxicity and enzyme assays. [Results] The interaction between SPY and CHP caused an additive effect in increasing the larvicidal activity of SPY∶CHP(1∶9) and asignificant synergistic effect in improving the larvicidal activity of SPY∶CHP(9∶1). Moreover, SPY∶CHP(1∶9) showed higher inhibitory activity on ACh E and Car E, whereas SPY ∶CHP(9 ∶1) revealed a significant stronger inhibitory effect on Car E and a similar inhibitory effect on ACh E compared with the effect of chlorpyrifos. [Conclusions] The induction of SPY can enhance the inhibitory activity of chlorpyrifos against ACh E and Car E in M. separata larvae.

  • 【分类号】S482.3
  • 【被引频次】1
  • 【下载频次】97
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