【摘要】 [目的]建立一种反相高效液相色谱测定吡蚜酮原药的定量分析方法。[方法]采用高效液相色谱法,使用Acclaim C₁₈色谱柱,以乙腈与0.2 g/L磷酸二氢钾缓冲盐溶液（体积比10∶90）为流动相,流速为1.0 m L/min,紫外检测器检测波长为250 nm,对吡蚜酮原药进行分离和测定。[结果]当吡蚜酮质量浓度为40～600 mg/L时,吡蚜酮的线性相关系数为0.9999,标准偏差为0.31;变异系数为0.31%,平均回收率为99.9%。[结论]该方法准确,快速,分离效果好,适合于吡蚜酮原药的定量分析。更多还原

【Abstract】 [Aims] A RP-HPLC method was established for the quantitative determination of pymetrozine TC.[Methods] The content of active ingredient in pymetrozine TC was quantitatively determined by HPLC with the Acclaim C₁₈ chromatographic column, and acetonitrile-0.2 g/L potassium dihydrogen phosphate buffer solution（10∶90,by vol） was used as eluent at the flow rate of 1 m L/min. The wave length of UV detector was 250 nm. [Results] When the concentration of pymetrozine range from 40 to 600 mg/L, the linear correlation coefficient was 0.9999, the standard deviation was 0.31, the variation coefficient was 0.31%, and the mean recovery was 99.9%. [Conclusions] The method is simple, accurate and suitable for quantitative analysis of pymetrozine.更多还原