【摘要】 目的探讨双孔钾离子通道TASK-1、TRAAK在精子中的表达水平及TASK-1、TRAAK在弱精子症发生机制中的作用。方法采用非连续密度梯度离心分离、纯化正常对照组精液标本20例和弱精子症组精液标本30例,提取精子总RNA后,采用RT-PCR,SYBR Green实时定量PCR检测精子中TASK-1、TRAAK mRNA的相对表达量。结果 TASK-1、TRAAK mRNA在正常对照组和弱精子症组精子中均有表达;其中,TRAAK在弱精子症患者精子中的表达量明显低于其在正常对照组精子中的表达量,下降2.3倍,两组之间有明显的统计学差异(P<0.05),TASK-1在弱精子症患者精子中的表达量与其在正常对照组精子中的表达量没有明显差异(P>0.05)。结论 TRAAK基因在弱精子症患者精子中的表达下调可能与精子活力下降有关,提示TRAAK可能成为弱精子症发病机制研究的一个分子靶标。更多还原

【Abstract】 Objective To investigate the expression level of K2 P channel TASK-1,TRAAK in the sperm cells and the role of TASK-1,TRAAK in the pathogenesis of asthenozoospermia.Methods Semen samples were collected from20 normal controls and 30 asthenozoospermia patients and purified using Percoll discontinuous density gradients;after extracted the total RNA,the relative expressions of TASK-1,TRAAK mRNA were detected in the two groups by RT-PCR,SYBR Green real-time PCR.Results TASK-1,TRAAK mRNA were expressed in the sperm cells both in the normal control group and the asthenozoospermia patient group;the expression of TRAAK mRNA was down-regulated by 2.3 times in the asthenospermia patients,significantly lower than in the normal control group(P<0.05),while,there was no significant difference of TASK-1 mRNA in the two groups(P>0.05).Conclusion The down-regulation of TRAAK mRNA in the sperm of asthenospermia patients may be related with decreased sperm motility,which suggests that TRAAK may serve as a potential molecular target for the research of asthenospermia.更多还原