【摘要】 目的 :探讨穹窿主体蛋白(major vault protein,MVP)对血管平滑肌细胞(vascular smooth muscle cells,VSMCs)增殖的影响。方法:分离培养野生型小鼠VSMCs,用血小板源性生长因子(PDGF-BB)或15%胎牛血清(FBS)刺激VSMCs增殖,Western blot检测VSMCs的MVP表达水平,同时检测特异性分化标记物α-SMA和SM22α表达水平。然后用PDGF-BB刺激人主动脉平滑肌细胞(HAo SMCs),检测MVP的表达差异。再分别分离培养野生型小鼠和MVP敲除型小鼠的VSMCs,通过CCK8细胞增殖实验检测两者的增殖能力;同时用si RNA干扰的方法,使HAo SMCs中MVP低表达,检测其增殖能力的变化。结果:用PDGFBB或15%FBS刺激VSMCs后,MVP表达水平呈浓度和时间依赖性下降,α-SMA和SM22α表达水平也相应降低。MVP缺失或低表达情况下,VSMCs的增殖能力均下降。结论:MVP在VSMCs增殖中起了重要作用。更多还原

【Abstract】 Objective:To investigate the effects of major vault protein(MVP)on the proliferation of vascular smooth muscle cells(VSMCs). Methods:The wild-type mouse VSMCs were isolated,and cultured,and the human aortic smooth muscle cells(HAo SMCs)were used. Western blot analysis was used to determine the MVP,α-SMA and SM22α. Expression of MVP stimulated by plateletderived growth factor(PDGF-BB)or 15% fetal bovine serum(FBS) was measured. MVP si RNA was used to downregulate the MVP expression in HAo SMCs. Cell proliferation CCK-8 assay was conducted to detect the proliferation ability of VSMCs in the case of MVP deficiency or downregulation. Results:After the treatment of PDGF-BB or 15% FBS,the MVP expression of VSMCs was downregulated in a time-dependent and dose-dependent manner. Compared with wild-type mouse VSMCs,the proliferation of MVP in knockout mouse VSMCs was suppressed. MVP si RNAs inhibited FBS-induced HAo VSMCs proliferation. Conclusion:MVP may played crucial effects in the proliferation of the VSMCs.更多还原