[Objectives]In order to know the situation of soybean seed-borne pothogens in Huang-huai area,the soybean seed-borne pathogens were detected using loop-mediated isothermal amplification( LAMP) assays. [Methods]In this research,13 species of seed-borne pothogens on soybean seed samples of 29 cultivars in Huang-huai area were detected using LAMP method,including Colletotrichum truncatum,C. gloeosporioide,Fusarium oxysporum,F. equiseti,F.proliferatum,F. culmorum,F. graminearum,F. solani,Phomapsis longicolla,Rh...