高效液相色谱-串联质谱法测定猪血中环丙沙星

【摘要】 建立高效液相色谱-串联质谱法（ultra performance liquid chromatography-tandem mass spectrometry,UPLC-MS/MS）测定猪血中环丙沙星的方法。样品中的环丙沙星用乙腈提取,UPLC-MS/MS进行分析。分析时采用Waters BEH C₁₈色谱柱（100mm×2.1 mm,1.7μm）,以0.10%甲酸水-甲醇作为流动相进行梯度洗脱,电喷雾正电子（ESI+）模式电离,多反应监测模式检测,外标法进行定量。结果表明:环丙沙星标准溶液在1.0⁵0μg/L范围内,峰面积与含量呈线性相关。环丙沙星的检出限为0.15μg/kg,样品中添标回收率在86%¹01%之间,相对标准偏差小于10%。更多还原

【Abstract】 A simple and sensitive Ultra Performance Liquid Chromatography-Tandem Mass（UPLC-MS/MS） method for the determination of Ciprofloxacin in swine blood was developed. Samples were extracted with acetonitrile and analyzed by UPLC-MS/MS on a Waters Acquity BEH C₁₈ column with 0.10% formic acid in water/methanol as the mobile phase by means of gradient elution. Detection was carried out with an electrospray ionisation（ESI） probe and operated in the positive ion mode. Ciprofloxacin was quantified by the external standard method. There was a good linearity in the range of concentrations between 1.0 and 50.0 μg/L. The limits of detection for Ciprofloxacin was 0.15μg/L. The recoveries from spiked samples were in the range of 86% to 101% and the relative standard deviations were lower than 10%.更多还原