【摘要】 目的了解河南省无偿献血人群HLA抗体的分布和特异性,评估无偿献血中因含有HLA抗体引起免疫性输血不良反应的风险。方法随机收集河南红十字血液中心已育女性血浆标本140(人)份、未育女性标本94(人)份,男性标本80(人)份,采用HLA抗体检测试剂盒对标本HLA抗体进行检测,阳性者进一步鉴定抗体特异性。结果 314名无偿献血者中,已育女性献血者HLA抗体检出阳性率27.86%(39/140),而未育女性献血者和男性献血者的阳性率仅为2.12%(2/94)和1.25%(1/80)。已育女性献血者中,HLA抗体出现频率较高的为抗A*23∶01、抗B*13∶01、抗B*13∶02、抗C*17∶01、抗DRB1*09∶01、抗DRB3*01∶01、抗DRB5*01∶01。结论已育女性献血者HLA抗体检出阳性率明显高于其他人群,为指导临床用血安全和制定相关政策提供了理论依据。更多还原

【Abstract】 To identify the distribution of HLA antibodies among voluntary blood donors in Henan province and estimate the risk of immune-mediated transfusion related adverse reaction caused by HLA antibodies,140 parous females,94 nulliparous females and 80 males were collected randomly from volunteer blood donors in Henan province.HLA antibodies from blood samples were detected using ELISA kit,and the positive samples were further determined for HLA genotypes.The results showed that among the 314 volunteer blood donors,the positive rate of HLA antibodies was 27.86%in parous females,significantly higher than that in nulliparous females and males(2.12%and 1.25%,respectively).The most frequently present HLA antibodies were anti-A*23:01,anti-B*13:01,anti-B*13:02,anti-C*17:01,anti-DRBl*09:01,anti-DRB3*01:01,and anti-DRB5*01:01 in parous females.These data provide practical basis for safe transfusion and relevant policy making.更多还原