【摘要】 目的对Buerger’s病患者血管中的髓样分化因子(myeloid differentiation factor88,My D88)进行检测,观察My D88在Buerger’s病患者血管中的表达及分布。方法收集2013年7月至2015年7月我院Buerger’s病患者血管标本9例,同期非Buerger’s病(肿瘤、外伤)患者血管标本12例为对照组。运用RT-PCR、Western blot技术检测2组血管中My D88的表达水平。同时运用免疫组化、免疫荧光技术检测Buerger’s病血管中My D88的表达及分布规律。结果 RT-PCR、Western blot结果显示,与对照组相比,Buerger’s病患者血管组织中My D88的m RNA和蛋白表达水平明显升高,具有显著统计学意义(P<0.01)。免疫组化、免疫荧光结果显示,在Buerger’s病患者血管组织中,My D88主要表达于内皮细胞和平滑肌细胞。结论 Buerger’s病患者血管组织中My D88基因和蛋白表达水平明显升高,且主要表达于血管平滑肌细胞和内皮细胞。这些结果表明My D88可能与Buerger’s病的发病密切相关,TLRs-My D88信号通路在Buerger’s病的发病机制中可能发挥着重要作用。更多还原

【Abstract】 To compared the difference of the expression and distribution of myeloid differentiation factor88(My D88) in the artery between Buerger’s disease and control, 9 cases patients with Buerger’s disease undergoingthe artery resection from July 2013 to July 2015 were collected as Buerger’s disease group, and 12 cases patientswith malignant tumor or injury undergoing the artery resection were receuited as controls. The m RNA and proteinlevels of My D88 were measured using the methods of RT-PCR and Western blot, respectively. In addtion, Thecharacteristics of My D88’s distribution was observed using the methods of immunohistochemistry andimmunofluorescence. According to the results of RT-PCR and Western blot, the level of My D88 expressed in theartery tissues of Buerger’s disease was obviously higher than that in the control tissues(P<0.01); the results ofimmunohistochemistry and immunofluorescence further showed the m RNA and protein levels of My D88 werestrongly up-regulated in the arteries with Buerger’s disease as compared with controls, and the My D88 proteinprimarily distributed in the arterial smooth muscle cells and the endothelial cells in Buerger’s disease. These resultsindicate that My D88 is probably related to pathogenesis of Buerger’s disease and the signaling pathway ofTLRs-My D88 maybe play a key role in the development of Buerger’s disease.更多还原