In order to explore the effect of exogenous chromosome or fragment on grain filling and yield of wheat,the offsprings ofNingchun 4× Lolium perennedistant hybridization were used as materials in this study.The chromosome composition of BC1 hybrid offsprings was identified based on genomic in situ hybridization(GISH)using Lolium perenne L.DNA as probe and molecular markers,and characteristics of grain filling and yield traits in BC1F3 offsprings were analyzed.The results showed that,No.ND99 had two GISH signa...