【摘要】 目的了解矢车菊色素对高龄2型糖尿病大鼠(Goto-Kakizaki rats,GK大鼠)肾损害时B细胞淋巴瘤伯血病基因-2(B cell lymphoma/lewkmia-2,Bcl-2)、Bcl-2相关X蛋白(Bcl-2 assaciated X protem,Bax)基因表达的影响。方法 25周龄雄性GK大鼠24只随机分为矢车菊组和对照组,每组12只。矢车菊组饲喂含10%葡萄糖的矢车菊色素-3-葡萄糖苷溶液600 mg/L8周,对照组饲喂10%葡萄糖溶液8周。每周测量体重及空腹血糖,8周后处死。RT-qPCR检测大鼠左肾Bcl-2、Bax mRNA表达,同时检测左肾脏器指数、血清糖化血红蛋白(glycated hemoglobin A1c,GHbA1c)并观察右肾病理损害。结果矢车菊组肾Bcl-2 mRNA表达高于对照组,差异有统计学意义(P<0.05)。矢车菊组Bax基因与对照组比,mRNA的表达差异无统计学意义(P>0.05)。矢车菊组GK大鼠的体重、空腹血糖、血清GHbA1c和左肾脏器指数较对照组均降低(P<0.05)。与对照组比较,矢车菊组肾脏病理损害明显减轻。结论矢车菊色素可降低GK大鼠的体重、空腹血糖和血清GHbA1c水平,减轻糖尿病的肾损伤,可能与促进Bcl-2基因表达有关。更多还原

【Abstract】 Objective To investigate the influence of cyanidin-3-glucoside on gene expression of Bcl-2 and Bax in kidney lesion of old GK rats.Methods 24 healthy male GK rats were divided randomly into two groups:blank control group(n = 12) and cyanidin group(n = 12).cyanidin group was fed in 10%glucose with cyanidin-3-glucoside(600 mg/L).The blank control group was fed in 10%glucose only.The weight,fasting plasma glucose(FPG) were detected each week.8 weeks later,all GK rats were killed.The mRNA expression of Bcl-2 and Bax was detected by RT-qPCR.The left kidney weight index,serum GHbAlc and histopathologicalchange of right kidney were examined too.Results The mRNA expression of Bcl-2 was higher than the blank control group(P<0.05).No statistical significance of the Bax mRNA expression were detected between the cyanidin group and blank control(P>0.05).The weight,FPG,serum GHbAlc and left kidney weight index in cyanidin group were lower than the blank control group(P< 0.05).The renal? pathologic lesion in cyanidin group was lighter than the blank control group.Conclusion The weight,FPG,GHbAlc and kidney diabetes lesion of GK rats can be decreased by cyanidin-3-glucoside perhaps through the increasing of Bcl-2 mRNA expression.更多还原