【摘要】 目的评估一种基于显微计数新方法(ADAM-r WBC)在残余白细胞定量检测中的性能和应用可行性。方法以Sysmex KX21全自动血球计数法及Nageotte血细胞计数板法作为参比,分析ADAM-r WBC检测白细胞的定量准确性和可重复性,并评估其在成分血白细胞残留检测中的实际性能。结果对WBC浓度分别为40、10和1 cell/μl的3份滤白红细胞悬液样品进行平行检测,ADAM-r WBC的批间变异系数分别为8.8、11.3和31.1,准确度分别为100.0%、87.5%和37.5%;而Nageotte计数板法的批间变异系数则分别为20.4、31.8和15.3,准确度分别为50.0%、12.5%和37.5%。对36份全血标本(白细胞浓度范围为4辱与共4 500~10 700 cell/μl),采用ADAM-r WBC进行稀释定量与采用Sysmex kx21进行直接计数的结果间差异无统计学意义(P=0.655),且线性相关性较好(Slope=1.00±0.01,R2=0.832,P<0.001)。对34份滤白红细胞悬液进行残留白细胞测定,平行采用ADAM-r WBC和Nageotte计数法所获结果间的差异无统计学意义(P=0.18),两种方法定量值均低于3.0 cell/μl,符合白细胞残余量<12.5 cell/μl的质控要求。结论采用ADAM-r WBC进行白细胞定量测定准确可靠,相比于Nageotte计数法重复性和精度更佳,能满足成分血白细胞残余量检测的国标需求。更多还原

【Abstract】 Objective Transfusion of blood components containing high amounts of leucocytes possibly causes side-effects in recipients. Therefore, the measurement of the residual leucocytes is certainly required for quality control of blood components. The study aimed to evaluate the performance of a new microscopic system(ADAM-r WBC) for residual leucocytes determination in blood components. Methods The quantitative accuracy, reproducibility and practical performance of ADAM-r WBC in detection of leucocytes was evaluated when compared with sysmex KX21 hematology analyzer and manual counting in the nageotte chamber. Results In a parallel detection of 3 specimens which contained 40, 10 and 1 cell/μl, the inter-assay coefficient of variation(CV %) was 8.8, 11.3, and 31.1 for ADAM-r WBC and 20.4, 31.8, and 15.3 for Nageotte. The accuracy was 100%, 87.5%, and 37.5% for ADAM-r WBC and 50%, 12.5%, and 37.5% for Nageotte, respectively. In the detection of 36 complete blood samples(white leucocytes counting ranged from 4500 to 10 700 cell/μl), the WBC quantification results from ADAM-r WBC diluting- measurement were comparable(P =0.655) and well-correlated(Slope=1.00±0.01, R 2=0.832, P <0.001) with that from Sysmex kx21 hematology analyzer. In detection of 34 blood components after leucocytes depletion, no significant difference(P =0.18) was observed between ADAM-r WBC and Nageotte chamber, all sample presenting a WBC amount <3.0 cell/ μl, which coincided with the standard requirement of <12.5 cell/μl. Conclusion The ADAM-r WBC was a accurate and reliable method for white leucocyte measurement. In comparison with manual counting, the ADAM-r WBC was more reproducible and was satisfied of convenience and be able to meet the national requrement.更多还原