【摘要】 目的探讨RUNX3基因多态性与胃癌的癌前病变——萎缩性胃炎(CGA)的关系。方法通过聚合酶链反应-限制性酶片段长度多态性(PCR-RFLP)对来自Hardy-Weinberg平衡群体的80例CGA和80例浅表性胃炎(CSG)患者RUNX3基因rs760805位点的基因型和等位基因频率进行分析。RUNX3基因rs760805位点的基因型和基因频率突变情况用χ2检验方法分析。通过非条件Logistic回归模型计算的风险值(OR)对rs760805位点各基因型与CGA发生风险度的关系进行分析。结果 CGA组RUNX3基因rs760805位点TT、TA和AA基因型的频率分别占20.0%、42.5%和37.5%,CSG组TT、TA和AA基因型的频率分别为35.0%、40.0%和25.0%,差异有统计学意义(P<0.05)。CGA组RUNX3基因rs760805位点等位基因A频率(58.75%)显著高于CSG组(45.00%)(P<0.05)。与RUNX3基因rs760805基因位点TT基因型相比,TA基因型、AA基因型和TA+AA基因型发生CGA的风险值分别为1.313(95%CI:0.940~1.833)、1.591(95%CI:1.060~2.389)和1.420(95%CI:1.051~1.918),P值分别为0.059、0.011和0.017。结论与RUNX3基因rs760805位点TT基因型相比,AA和TA+AA基因型显著增加了CGA发生的风险,与CGA的发生有一定的相关性。更多还原

【Abstract】 Objective To investigate the relationship between RUNX3 gene polymorphism and chronic gastric atrophy(CGA). Methods 80 CGA and 80 superficial gastritis(CSG) patients came from Hardy-Weinberg’s equilibrium. We used polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) to analyze the genotypes frequencies and alleles frequencies of RUNX3 rs760805. The differences between genotypes frequencies and alleles frequencies of RUNX3 rs760805 sites were analyzed by using χ2 test. The odds ratio(OR) of various genotypes were calculated by unconditional logistic regression model to assess the relationship between the rs760805 genotypes and the risk of CGA. Results The genotype frequencies of RUNX3 rs760805 TT, TA and AA in CGA were 20.0%, 42.5% and 37.5% respectively, and the frequencies of RUNX3 rs760805 TT, TA and AA in CSG were 35.0%, 40.0% and 25.0% respectively; there was a statistical difference between CGA and CSG(P<0.05). The frequency of RUNX3 gene rs760805 allele A in CGA(58.75%) was higher than that in CSG(45.00%)(P<0.05) significantly. The OR of genotype TA, AA and TA+AA is 1.313(95% CI: 0.940~1.833), 1.591(95% CI: 1.060~2.389) and 1.420(95% CI: 1.051~1.918) compared with TT genotype respectively. Conclusion RUNX3 rs760805 genotype AA and TA+AA increased the risk of CGA significantly and it may be associated with the pathogenesis of CGA.更多还原