【摘要】 目的:探索急性髓细胞白血病(AML)骨髓中Th17细胞及其相关因子在AML发生与发展中的作用。方法:选取初诊AML患者23例,另取25名健康志愿者为对照。采用流式细胞仪分别测初诊AML组、初诊AML组诱导缓解后、对照组骨髓中CD3~+ CD161~+ IL-17~+ IFN-γ~+ T细胞的比例。采用ELISA法检测初诊AML组、初诊AML组诱导缓解后、对照组骨髓液中血管内皮生长因子(VEGF)、IL-17浓度;对VEGF与IL-17进行相关性分析。结果:初诊AML组骨髓中CD3~+ CD161~+ IL-17~+ IFN-γ~+ T细胞的比例为(1.52±0.10)%,初诊AML组诱导缓解后CD3~+ CD161~+ IL-17~+ IFN-γ~+ T细胞的比例为(1.36±0.06)%,对照组骨髓中CD3~+ CD161~+ IL-17~+ IFN-γ~+ T细胞的比例为(0.22±0.04)%。初诊AML组VEGF浓度(254.08±24.34)pg/ml,IL-17浓度(20.24±3.82)pg/ml;初诊AML组诱导缓解后VEGF浓度(137.37±22.51)pg/ml,IL-17浓度(10.42±2.96)pg/ml;对照组骨髓液中VEGF浓度(125.98±14.96)pg/ml,IL-17浓度(10.59±2.87)pg/ml;VEGF与IL-17呈正相关。结论:Th17细胞具有促进AML发生与发展的作用。更多还原

【Abstract】 Objective:To explore the role of Th17 cells and related factors in acute myeloid leukemia(AML).Method:The ratio of CD3~+ CD161~+ IL-17~+ IFN-γ~+ T cells in bone marrow was examined by flow cytometry.Levels of VEGF,IL-17 in bone marrow were measured by ELISA.Result:The ratio of CD3~+ CD161~+ IL-17~+ IFN-γ~+ T cells in newly diagnosed AML patients was(1.52±0.10)%,which was similar to the results of leukemia after CR(1.36±0.06)%(P>0.05),and was higher than that in control group(0.22±0.04)%(P<0.05).Levels of VEGF,IL-17 in newly diagnosed AML patients were(254.08±24.34)pg/ml,(20.24±3.82)pg/ml,which were significantly higher than those in leukemia after CR(137.37±22.51)pg/ml,(10.42±2.96)pg/ml and control group(125.98±14.96)pg/ml,(10.59±2.87)pg/ml(P<0.05).And VEGF was positively related with IL-17.Conclusion:Th17cells may play apromoting role in AML evolution.更多还原