Objective:To establish immortalized B lymphoblastoid cell lines(iBLCLs)from peripheral blood mononuclear cells(PBMCs)of healthy human and immune thrombocytopenia(ITP)patients in vitro and study its biological function.Method:PBMCs were isolated from ITP patients and normal controls,and then incubated with Epstein-Barr virus(EBV).Then optical microscope was used to observe iBLCLs.The gene expression of EBV encoded latent membrane protein 1(LMP-1)in iBLCLs were detected by RT-PCR.The cell cycle and expression...
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