【摘要】 目的研究辛伐他汀对慢性阻塞性肺病大鼠基质金属蛋白酶-9(MMP-9)及其抑制剂(TIMP-1)表达的影响。方法选用30只健康的雄性SD大鼠作为实验材料,将30只大鼠分为三组:A组10只,B组10只,C组10只。B组和C组构建慢阻肺模型,C组大鼠采用辛伐他汀治疗。比较各组大鼠的一般症状、肺功能、支气管肺泡灌洗液(BALF)细胞计数和血清MMP-9、TIMP-1水平。结果 4w后,各组大鼠的体质量、FEV0.3s、FVC、FEV0.3s/FVC、PEV和BALF细胞计数具有统计学差异(P<0.05)。A组大鼠MMP-9为(87.14±21.76)ng/ml,TIMP-1为(82.16±14.89)ng/ml,MMP-9/TIMP-1为(1.07±0.09);B组大鼠MMP-9为(241.58±38.94)ng/ml,TIMP-1为(167.92±19.76)ng/ml,MMP-9/TIMP-1为(1.44±0.21);C组大鼠MMP-9为(134.25±29.15)ng/ml,TIMP-1为(119.41±15.62)ng/ml,MMP-9/TIMP-1为(1.12±0.1),差异具有统计学意义(P<0.05)。结论辛伐他汀可以降低慢阻肺大鼠基质金属蛋白酶-9及其抑制剂的表达水平,并改善两者的比例失衡。更多还原

【Abstract】 Objective To study the influence of simvastatin on matrix metalloproteinase-9（ MMP-9） and tissue inhibitor of metalloproteinase-1（ TIMP-1） in rats with chronic obstructive pulmonary disease. Methods 30 male SD rats were chosen as study objects,and all rats were divided into 3 groups: 10 in the group A（ control group）,10 in the group B（ model group） and 10 in the group C（ simvastatin）. After 4 weeks,the symptoms,pulmonary function,cell counting of BALF and level of MMP-9 and TIMP-1 were compared among the 3 groups. Results After 4 weeks,the body mass,FEV_{0. 3}s,FVC,FEV_{0. 3}s/ FVC,PEV and cell counting of BALF had statistic difference among the 3 groups（ P < 0. 05）. In the group A,MMP-9 was（ 87. 14 ± 21. 76） ng / ml,TIMP-1was（ 82. 16 ±14. 89） ng / ml,and MMP-9 / TIMP-1 was（ 1. 07 ± 0. 09）. In the group B,MMP-9 was（ 241. 58 ± 38. 94） ng / ml,TIMP-1 was（ 167. 92 ± 19. 76） ng / ml,and MMP-9 / TIMP-1 was（ 1. 44 ± 0. 21）. In the group C,MMP-9 was（ 134. 25 ± 29. 15） ng/ml,TIMP-1 was（ 119. 41 ± 15. 62） ng/ml,and MMP-9 /TIMP-1 was（ 1. 12 ± 0. 1）. The difference had statistical significance（ P < 0. 05）. Conclusion Simvastatin can reduce the expression level of MMP-9 and TIMP-1 in rats with COPD,as well as regulate their ratio.更多还原