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辛伐他汀对慢阻肺大鼠MMP-9及其抑制剂表达的影响
Influence of simvastatin on MMP-9 and tissue inhibitor of metalloproteinase-1 in rats with chronic obstructive pulmonary disease
【摘要】 目的研究辛伐他汀对慢性阻塞性肺病大鼠基质金属蛋白酶-9(MMP-9)及其抑制剂(TIMP-1)表达的影响。方法选用30只健康的雄性SD大鼠作为实验材料,将30只大鼠分为三组:A组10只,B组10只,C组10只。B组和C组构建慢阻肺模型,C组大鼠采用辛伐他汀治疗。比较各组大鼠的一般症状、肺功能、支气管肺泡灌洗液(BALF)细胞计数和血清MMP-9、TIMP-1水平。结果 4w后,各组大鼠的体质量、FEV0.3s、FVC、FEV0.3s/FVC、PEV和BALF细胞计数具有统计学差异(P<0.05)。A组大鼠MMP-9为(87.14±21.76)ng/ml,TIMP-1为(82.16±14.89)ng/ml,MMP-9/TIMP-1为(1.07±0.09);B组大鼠MMP-9为(241.58±38.94)ng/ml,TIMP-1为(167.92±19.76)ng/ml,MMP-9/TIMP-1为(1.44±0.21);C组大鼠MMP-9为(134.25±29.15)ng/ml,TIMP-1为(119.41±15.62)ng/ml,MMP-9/TIMP-1为(1.12±0.1),差异具有统计学意义(P<0.05)。结论辛伐他汀可以降低慢阻肺大鼠基质金属蛋白酶-9及其抑制剂的表达水平,并改善两者的比例失衡。
【Abstract】 Objective To study the influence of simvastatin on matrix metalloproteinase-9( MMP-9) and tissue inhibitor of metalloproteinase-1( TIMP-1) in rats with chronic obstructive pulmonary disease. Methods 30 male SD rats were chosen as study objects,and all rats were divided into 3 groups: 10 in the group A( control group),10 in the group B( model group) and 10 in the group C( simvastatin). After 4 weeks,the symptoms,pulmonary function,cell counting of BALF and level of MMP-9 and TIMP-1 were compared among the 3 groups. Results After 4 weeks,the body mass,FEV0. 3s,FVC,FEV0. 3s/ FVC,PEV and cell counting of BALF had statistic difference among the 3 groups( P < 0. 05). In the group A,MMP-9 was( 87. 14 ± 21. 76) ng / ml,TIMP-1was( 82. 16 ±14. 89) ng / ml,and MMP-9 / TIMP-1 was( 1. 07 ± 0. 09). In the group B,MMP-9 was( 241. 58 ± 38. 94) ng / ml,TIMP-1 was( 167. 92 ± 19. 76) ng / ml,and MMP-9 / TIMP-1 was( 1. 44 ± 0. 21). In the group C,MMP-9 was( 134. 25 ± 29. 15) ng/ml,TIMP-1 was( 119. 41 ± 15. 62) ng/ml,and MMP-9 /TIMP-1 was( 1. 12 ± 0. 1). The difference had statistical significance( P < 0. 05). Conclusion Simvastatin can reduce the expression level of MMP-9 and TIMP-1 in rats with COPD,as well as regulate their ratio.
- 【文献出处】 临床肺科杂志 ,Journal of Clinical Pulmonary Medicine , 编辑部邮箱 ,2016年06期
- 【分类号】R563.9
- 【被引频次】2
- 【下载频次】102