Objective:To study the effect of MT01 on the differentiation of osteoblasts infected by porphyromonas gingivalis(Pg).Methods:The MG63 cells were seeded into 6-well cell culture plates.MT01 at a final concentration of 1μg·mL-1 was added and incubated for 3hours.Then the cells were challenged by Pg(MOI=100∶1).There were four experimental groups:blank group,MT01,Pg and MT01+Pg.ALP activities in the supernatant fluid and the cell lysate after 24 hours were detected by ALP kit.Real-time PCR was used to detect th...
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