【摘要】 目的:研究慢性应激对小鼠空间学习记忆的影响及机制。方法 :慢性应激模型的建立采用的是持续21天的7种不同应激方式,通过Morris水迷宫实验检测小鼠的空间学习记忆能力,免疫组织化学方法检验小鼠脑内各脑区wnt3a的表达。结果:慢性应激组小鼠与对照组小鼠相比,在定位航行试验中,小鼠寻找平台的逃避潜伏期明显提高(P<0.05),空间搜索实验中,应激组小鼠在目标象限的游泳时间明显减少(P<0.05)。在小鼠脑内PFC区wnt3a表达显著减少(P<0.01)。结论 :慢性应激对小鼠空间学习记忆的能力具有损伤作用,这与wnt3a在脑内的表达关系密切。更多还原

【Abstract】 Objective To study the effects of chronic stress on the spatial learning-memory function of mice and its mechanism. Method The chronic stress model of mice was induced by stimulation of multiple stressors for 21 days. The ability of spatial learning-memory of mice were determined by Morris Water maze task, and the expression of Wnt3 a in the hippocampus and prefrontal cortex were detected by immunohistochemical method.Result After chronic stress, the stress group’s escape latency in the place navigation test were significantly higher than control group mice(P<0.05). In the spatial search test, after chronic stress,respectively compared with control group mice, in first quadrant(i.e. platform quadrant) the swimming time of stress group mice were obviously shortened(P<0.05). The Wnt3 a expression in PFC were significantly shortened in stress group mice(P<0.01). Conclusion The ability of chronic stress on spatial learning and memory in mice has the function of damage, which is closely related to the expression of Wnt3 a in brain.更多还原