【摘要】 目的比较代谢综合征(MS)患者及单纯高血压(EH)患者血单核细胞Toll样受体4(TLR4)及血浆高敏C反应蛋白(hs-CRP)的表达。方法采用流式细胞仪检测62例EH患者及45例MS患者血单核细胞TLR4的阳性率,同时检测血浆hs-CRP浓度。并将TLR4及hs-CRP分别与年龄、性别、总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白(HDL)、低密度脂蛋白(LDL)、空腹血糖(FBG)、糖化血红蛋白(Hb A1c)、TLR4、hs-CRP行单因素相关分析,然后进行多元逐步回归分析。结果 MS组TLR4(5.7%±1.2%)、hs-CRP(5.6±0.9 mg/L)与单纯EH组(分别为5.3%±0.9%、5.0±0.8 mg/L)患者差异均有统计学意义(t值分别为-3.274、-2.225,P值分别为0.028、0.01);单因素相关分析显示,TLR4和hs-CRP均与TC、LDL、Hb A1c、FBG、餐后2 h血糖(2h PBG)呈正相关(P<0.05),TLR4与hs-CRP也呈正相关(P<0.05);多元逐步回归分析显示,影响TLR4最显著因素为hs-CRP、Hb A1c(β值分别为0.745、0.244,P值分别为0.000、0.004),影响hs-CRP最显著因素为TLR4(β=0.943,P=0.000)。结论MS患者单核细胞TLR4阳性率、血浆hs-CRP均较单纯EH患者高,MS患者单核细胞TLR4阳性率与血浆hsCRP水平呈正相关,TLR4可能参与了MS发生和发展。更多还原

【Abstract】 Aim To compare the expression of Toll like receptor 4( TLR4) in blood mononuclear cells and plasma high-sensitivity C-reactive protein( hs-CRP) between metabolic syndrome( MS) patients and simple hypertensive patients. Methods There were 62 EH patients and 45 patients with MS. The TLR4 positive rate in blood mononuclear cells was detected by flow cytometry. We also detected the plasma hs-CRP of the patiens. Single factor analysis was performed on TLR4,hs-CRP and other observation indexes( age,sex,total cholesterol( TC),triglyceride( TG),high density lipoprotein( HDL),low density lipoprotein( LDL),fasting blood glucose( FBG) and glycated hemoglobin( Hb A1c)),and then multi-factor regression analysis was also performed. Results TLR4 and hs-CRP were statistically significant between MS group( 5. 7% ± 1. 2% and 5. 6 ± 0. 9 mg / L) and EH group( 5. 3% ± 0. 9% and 5. 0 ± 0. 8mg / L)( t values were- 3. 274,- 2. 225,P values were 0. 028,0. 01); Single factor analysis showed that TLR4 was positively correlated with TC,LDL,Hb A1 c,FBG,2-hour postprandial blood glucose( 2h PBG) and hs-CRP( P < 0. 05),and hs-CRP was positively correlated with TC,LDL,Hb A1 c,FBG,2h PBG,TLR4( P < 0. 05); Meanwhile,multi-factor regression analysis showed that hs-CRP and Hb A1 c were the most significant factors which affected TLR4( β values were0. 745,0. 244,P values were 0. 000,0. 004),and TLR4 was the most significant factor which affected hs-CRP( β =0. 943,P = 0. 000). Conclusion It is MS patients that have higher TLR4 positive rate in mononuclear cells and plasma hs-CRP compared with EH patients. TLR4 positive rate and plasma hs-CRP levels of MS patients are positively correlated. And TLR4 may be involved in the development and progression of MS.更多还原