【摘要】 目的克隆低等无脊椎环节典型代表动物日本刺沙蚕中枢神经系统色氨酸羟化酶基因全长,为无脊椎动物芳香族氨基酸羟化酶的基因进化研究提供依据。方法应用c DNA末端快速扩增(rapid amplification of c DNA ends,RACE)技术进行色氨酸羟化酶基因序列克隆。结果在日本刺沙蚕中枢神经系统克隆出色氨酸羟化酶基因3’序列和5’序列。结论在低等环节动物日本刺沙蚕中枢神经系统存在独立的色氨酸羟化酶基因,使色氨酸羟化酶和苯丙氨酸羟化酶基因分化的节点向前推进到环节动物。更多还原

【Abstract】 Objective To Clone tryptophan hydroxylase gene in central nervous system of nereis( N. japonica),and provide evidence for the study of aromatic amino acid hydroxylase gene evolution in invertebrate. Methods Rapid amplification of c DNA ends( RACE) was used to clone tryptophan hydroxylase gene. Results 3’ sequence and 5’ sequence of tryptophan hydroxylase gene were cloned in central nervous system of nereis( N. japonica). Conclusion Tryptophan hydroxylase gene exists in the central nervous system of Nereis( N. japonica) independently,which carries forward the node of tryptophan hydroxylase and phenylalanine hydroxylase gene differentiation to annelid.更多还原