Objective To investigate the characteristics, diagnosis and treatment of perineal endometriosis(PEM). Methods Clinical data about 12 patients who were diagnosed with PEM from January 2010 to December 2014 in Chinese PLA General Hospital were retrospectively analyzed. A range of 6–59 months of outpatient follow-up after surgery was conducted for these 12 patients. Results All12 patients were fertile woman with average age of 31.7 years, and they all had a history of perineotomy or vaginal laceration during v...