【摘要】 目的观察硫化氢（H₂S）对百草枯（PQ）中毒大鼠急性肺损伤（ALI）及炎症介质的影响。方法将30只雄性SD大鼠随机分为3组,每组10只,正常对照组（A组）,PQ中毒组（B组）,NaHS干预组（C组）。B、C组采用PQ灌胃（80mg/kg）复制大鼠PQ中毒肺损伤模型,B组同时腹腔注射生理盐水（6ml/kg）,每日两次,C组PQ染毒后,同时腹腔注射NaHS（1.4umol/kg）,每日两次;A组采用等量生理盐水灌胃,同时腹腔注射生理盐水（6ml/kg）,每日两次。72h后取血检测肿瘤坏死因子-а（TNF-а）、白细胞介素-6（IL-6）、白细胞介素-8（IL-8）的含量;在光学显微镜下观察肺组织病理学改变,进行肺损伤病理评分。结果 B组中TNF-а、IL-6、及IL-8含量明显高于A组（P<0.01）;C组中TNF-а、IL-6、及IL-8含量较B组明显减低（P<0.05）;B组大鼠肺损伤病理评分高于A组（P<0.01）;C组大鼠肺损伤病理评分较B组减低（P<0.01）。结论 H₂S具有抑制百草枯中毒大鼠炎症介质表达,减轻大鼠急性肺损伤作用。更多还原

【Abstract】 Objective To investigate the effect of hydrogen sulfide（H₂S） on acute lung injury（ALI） and inflammatory factors in rats with paraquat poisoning. Methods 30 male SD rats were randomly divided into 3 groups,10 rats in each group,normal control group（A group）,PQ poisoning group（B group）,NaHS intervention group（C group）. B,C,group of rats were made into the model of lung injury by pouring PQ（80mg/Kg） into the stomach,B group was injected with normal saline（6ml/kg） at the same time,two times a day. C group rats were exposed to PQ,while intraperitoneal injection of NaHS（1.4μmol/kg）,two times a day. In group A,the same amount of normal saline was used to fill the stomach,normal saline（6ml/kg） was injected by intraperitoneal at the same time,two times a day. Determinating the concentration of tumor necrosis factor-а（TNF-а）,interleukin-6（IL-6）,interleukin-8（IL-8） in peripheral blood after 72 hours. Observing the pathological changes of lung tissue,counting lung injury score under optical microscope. Results In group B,TNF-а,IL-6,and IL-8 were significantly higher than that of A group（P<0.01）. In group C,TNF-а,IL-6,and IL-8 was significantly lower than that of group B（P<0.05）. The lung injury score in B group was significantly higher than that in A group（P <0.01）. The lung injury score in the C group was significantly lower than that in the B group（P<0.01）. Conclusion H₂S can inhibit the expression of inflammatory mediators in rats with paraquat poisoning,and reduce the acute lung injury in rats.更多还原