【摘要】 目的探讨甲基转移酶抑制剂5-氮-2-脱氧胞苷(DAC)对胆管癌细胞TFK-1侵袭力的影响及其机制。方法采用Transwell法检测胆管癌细胞在不同浓度DAC(0、10、20、40、80、160μmol·L-1)作用下侵袭力的变化;Western-blot法检测DAC作用后Wif-1蛋白的表达。结果 DAC对胆管癌细胞侵袭力有显著性抑制(P<0.05),且呈浓度依赖性;Wif-1蛋白在TFK-1细胞中未见表达,DAC处理后Wif-1蛋白再现表达。结论 DAC对胆管癌TFK-1细胞的侵袭有抑制作用,此作用可能是由Wif-1基因去甲基化来实现。更多还原

【Abstract】 Objective To investigate the effect of DNA methyltransferase inhibitor 5-aza-2-deoxycytidine(DAC)on the invasion of TFK-1cholangiocarcinoma cells and its mechanism of action.Methods Transwell method was used to detect the invasion of cholangiocarcinoma cells treated with different concentrations of DAC(0,10,20,40,80 and 160μmol·L-1).Western-blot method was used to detect the expression of Wif-1protein.Results DAC inhibited the invasion of cholangiocarcinoma cells in a concentration-dependent manner.The expression of Wif-1protein was not observed in cholangiocarcinoma cells,but was detected after treatment with DAC.Conclusion DAC can inhibit the invasion of cholangiocarcinoma cells,and the mechanism may be involved in the demethylation of Wif-1gene.更多还原