【摘要】 目的探索乳腺癌间质成纤维细胞对乳腺癌作用的分子机制,研究与人乳腺癌细胞共培养的人成纤维细胞特异蛋白-l(fibroblast-specific protein-1,FSP-l)的表达及对乳腺癌生长的影响。方法 Transwell方法共培养人乳腺癌细胞株MDA-MB-231和人成纤维细胞株ESF;实时RT-qPCR检测共培养的MDA-MB-231和ESF细胞FSP-1mRNA水平;免疫荧光流式细胞仪检测共培养MDA-MB-231和ESF细胞FSP-1蛋白表达水平;Cell Counting Kit-8(CCK-8)检测MDA-MB-231细胞的增殖;免疫荧光激光共聚焦显微镜观察荷瘤裸鼠乳腺癌组织FSP-1的表达,检测FSP-1对荷瘤裸鼠肿瘤体积的影响。结果 MDA-MB-231细胞和ESF细胞共培养可上调ESF细胞表达FSP-1并促进MDA-MB-231细胞增殖;ESF+MDA-MB-231组MDA-MB-231细胞表达FSP-1,但在MDA-MB-231组未检测到FSP-1蛋白表达;荷瘤裸鼠MDA-MB-231细胞和ESF细胞共生长的癌组织高表达FSP-1并促进肿瘤生长。结论与人乳腺癌细胞共培养可上调人成纤维细胞FSP-1的表达并促进乳腺癌生长。更多还原

【Abstract】 Objective To investigate the molecular mechanisms of action of stromal fibroblasts on breast cancer,and to study the expression of fibroblast-specific protein-1(FSP-1)and its impact on breast cancer growth.Methods Human breast cancer MDA-MB-231 cells and human endometrial stromal fibroblasts(ESF)were co-cultured in Transwell system,or cultured alone.The expression of FSP-1mRNA was examined in MDA-MB-231 cells and ESF by RT-qPCR.The expression of FSP-1protein was detected by immunofluorescence flow cytometry.The proliferation of MDA-MB-231 cells was tested using Cell Counting Kit-8.The expression of FSP-1in breast cancer tissue of tumor-burdened nude mice was analyzed using immunofluorescence confocal laser microscopy,and the effect of FSP-1on tumor volume was examined.Results The expression of FSP-1was up-regulated in ESF co-cultured with MDA-MB-231 cells,and the up-regulation of FSP-1promoted the proliferation of MDA-MB-231 cells.FSP-1was expressed in MDA-MB-231 cells co-cultured with ESF,but not in MDA-MB-231 cells cultured alone.In addition,FSP-1was highly expressed in the cancer tissue with co-growth of MDA-MB-231 cells and ESF in nude mice,and the high expression of FSP-1accelerated tumor growth.Conclusion The co-culture with human breast cancer cells can up-regulate FSP-1expression and promote breast cancer growth.更多还原