【摘要】 目的探讨桑色素（morin,Mor）对全氟辛酸（perfluorooctanoic acid,PFOA）诱导小鼠肝损伤的保护作用。方法将24只雄性小鼠按随机数字表法分为4组:对照组（等容量生理盐水）、PFOA组(PFOA 10 mg·kg^-1·d^-1)、Mor组(Mor 75mg·kg^-1·d^-1)和PFOA+Mor组(PFOA 10mg·kg^-1·d^-1+Mor 75mg·kg^-1·d^-1),每组6只。连续灌胃14d。分别检测血清谷丙转氨酶、谷草转氨酶和碱性磷酸酶的活性,肝组织匀浆中丙二醛、C-反应蛋白、白细胞介素-6的水平,超氧化物歧化酶、过氧化氢酶和环氧化酶-2的活性。结果与对照组比较,PFOA组、PFOA+Mor组小鼠血清谷丙转氨酶、谷草转氨酶、碱性磷酸酶活性及PFOA组小鼠肝组织匀浆中丙二醛、C-反应蛋白、白细胞介素-6水平和环氧化酶-2活性均明显升高,PFOA组、PFOA+Mor组小鼠肝组织匀浆中超氧化物歧化酶、过氧化氢酶活性均明显降低（均P<0.05）;与PFOA组比较,Mor组、PFOA+Mor组小鼠血清谷丙转氨酶、谷草转氨酶和碱性磷酸酶活性,肝组织匀浆中丙二醛、C-反应蛋白、白细胞介素-6水平和环氧化酶-2活性均明显降低,肝组织匀浆中超氧化物歧化酶、过氧化氢酶活性均明显升高（均P<0.05）。结论桑色素对PFOA诱导的小鼠肝损伤具有保护作用,其机制可能与抑制氧化应激和炎症反应有关。更多还原

【Abstract】 Objective To investigate the protective effect of morin（Mor）on perfluorooctanoic acid（PFOA）-induced liver injury in mice.Methods Twenty-four male mice were randomly divided into four groups:control group（same volume of normal saline）,PFOA group(PFOA 10mg·kg^-1·d^-1),Mor group(Mor 75mg·kg^-1·d^-1)and PFOA+Mor group(PFOA 10mg·kg^-1·d^-1+Mor 75 mg·kg^-1·d^-1),with 6 mice in each group.After treatment for 14 consecutive days,serum activities of alanine transaminase（ALT）,aspartate transaminase（AST）and alkaline phosphatase（ALP）,hepatic contents of malondialdehyde（MDA）,and hepatic activities of superoxide dismutase（SOD）,catalase（CAT）,C-reactive protein（CRP）,interleukin-6（IL-6）and cyclooxygenase-2（COX-2）were measured in all groups.Results Compared with control group,liver injury indicators ALT,AST and ALP,inflammatory response markers CRP,IL-6and COX-2,and lipid peroxidation product MDA increased,but antioxidant enzymes SOD and CAT decreased in PFOA group（P<0.05）.Compared with PFOA group,simultaneous supplementation of Mor decreased serum activities of ALT,AST and ALP,reduced hepatic contents of MDA,CRP,IL-6and COX-2,and increased hepatic activities of SOD and CAT（P<0.05）.Conclusion Mor protects against PFOA-induced liver injury in mice through anti-oxidation and anti-inflammation.更多还原