【摘要】 采用RT-PCR方法克隆获得黄河鲤(Cyprinus carpio)金属硫蛋白基因的编码区(CDS)序列,该序列长183bp,编码60个氨基酸,不含芳香族氨基酸,其中20个半胱氨酸(Cys)集中分布在肽链的N端和C端,具有MT典型的Cys-X(1~3个)-Cys结构,预测分子量为6013 D,理论等电点为8.38。经多序列比对,黄河鲤与高身鲫(Carassius cuvieri)的同源性最高,达94%。采用RT-q PCR进行黄河鲤MT的组织表达分析,结果表明:黄河鲤MT基因的表达存在组织性差异,其相对表达量表现为肝脏>肾脏>腮>脑>肌肉。更多还原

【Abstract】 The CDS sequence of metallothionein( MT) gene in Cyprinus carpio was cloned by using RT-PCR. This sequence was 183 bp in full length,encoded 60 amino acid residues,and did not contain any aromatic amino acids. Twenty cysteine( Cys)were concentratedly distributed at the N terminal and C terminal of the peptide chain with the typical structure of Cys-X( 1 ~ 3 individuals)-Cys. Its molecular weight was predicted to be 6013 D,and theoretical isoelectric point was 8.38. In addition,the multiple sequence alignment showed that the MT gene in Cyprinus carpio shared the highest identity( 94%) with that in Carassius cuvieri. The tissue expression of MT gene in Cyprinus carpio was analyzed by using RT-q PCR,the results indicated that the relative expression level of MT gene in various tissues was different,and showed the following order: liver>kidney>gill >brain>muscle.更多还原