【摘要】 为了优化富硒荷叶离褶伞菌丝体胞内粗多糖发酵条件,在单因素试验基础上,考察灭菌条件、培养时间、硒添加量和硒添加时间对富硒荷叶离褶伞菌丝体胞内粗多糖的影响,并通过Box-Benhnken实验设计和响应面分析法确定最优发酵条件。结果表明,在121℃、20min条件下对培养基和Na2Se O3溶液分别灭菌后混合,在发酵至第129h,发酵液中添加4μg/m L Na2Se O3,继续发酵至第244h,发酵产生的富硒荷叶离褶伞菌丝体胞内粗多糖含量为349.8mg/100m L。更多还原

【Abstract】 One-factors-at-a-time experiments were carried out to investigate the effects of sterilizing condition, incubation duration, selenium addition amount and optimal replenishing time on the yield of crude intracellular polysaccharide of selenium-enrichded Lyophyllum decastes mycelia. The process parameters were optimized using a Box-Behnken experimental design coupled with response surface methodology. Culture medium and sodium arsenate solution were mixed after respective sterilization for 20 minutes under 121?C. It was showed that addition of 4μg/m L Na2 Se O3 in 129 h of incubation and continued extension of incubation duration resulted in high yield of crude intracellular polysaccharide in Lyophyllum decastes mycelia, reaching 349.8mg/100 m L in 244 h of fermentation.更多还原