【摘要】 目的研究氰戊菊酯(Fen)对雄性大鼠的时间生殖毒性。方法选择健康雄性SD大鼠36只,按给药时间分为日间染毒(9am)和夜间染毒(9pm)2类,每类随机分为对照组和高剂量(12mg/kg)、低剂量(2.4mg/kg)染毒组。对大鼠灌胃染毒30d,对照组给予等体积食用油。染毒前、后对大鼠体重进行称量,计算增长量;并在第31d分别取6个时间点(1am,5am,9am,1pm,5pm,9pm)的大鼠血清,应用酶联免疫法测定血清中睾酮(T)和雌二醇(E2)。结果氰戊菊酯染毒可使大鼠体重增长减慢,其血清中T和E2水平上升,与剂量均呈正相关(P值均<0.05)。染毒时间点对体重、血清中T和E2水平影响差异均无统计学意义(P值均>0.05)。正常大鼠血清T分泌具有昼夜节律(P<0.05),E2分泌无昼夜节律(P>0.05)。9am染毒的高、低剂量组大鼠T分泌的节律性均消失(P>0.05)。结论 Fen对雄性大鼠具有一定的生殖毒性,与剂量呈正相关;白天暴露较夜间暴露更敏感。更多还原

【Abstract】 Objective To study circadian chronotoxicity of fenvalerate(Fen)on reproductive hormones of male rats.Methods A total of 36 healthy SD male rats were randomly divided into daytime(9am)exposure group and night time(9pm)exposure group;each group was randomly divided into control sub-group which was treated with oil,high dose exposure sub-group(12mg/kg)and low dose exposure sub-group(2.4mg/kg)by intragastric adminstration for 30 d.Incensement of body weight was calculated.On 31 d,rats serum were collected at 6time points(1am,5am,9am,1pm,5pm,9pm)and analyzed for testosterone(T)and estradiol(E2)concentration by ELISA.Results Upon exposure of Fen,weight gain of rats was slowed down;while serum T and E2 increased,which were positively correlated with Fen dose(all P<0.05).No statistical difference was observed between daytime exposure group and night time exposure group for body weight,serum T and E2 levels in rats.The secretion of serum T of normal rats had circadian rhythm(P<0.05),while E2 had no circadian rhythm(P>0.05).Rats in daytime exposure group of both doses lost serum T rhythm(all P>0.05).Conclusion Fen was toxic on reproductive hormones in male rats,which was positively correlated with the dose;Rats were more susceptible to Fen when exposured on daytime.更多还原