【摘要】 以InsB15-23dtSCT原核表达载体为基础,制备dtSCT型IGRP206-214H-2Kd原核表达载体;将获取的融合蛋白IGRP206-214H-2Kd单体,生物素化制成IGRP206-214dtSCT四聚体,进一步将皂草素与IGRP206-214dtSCT四聚体偶联;流式检测皂草素偶联IGRP206-214dtSCT四聚体与NOD鼠脾脏淋巴细胞共孵育体系中IGRP206-214特异性细胞毒性T细胞(CTL)。结果表明:皂草素偶联IGRP206-214dtSCT四聚体与NOD鼠脾脏淋巴细胞共孵育后,IGRP206-214特异性CTL的频率与PBS对照组相比明显下降(P<0.001)。说明偶联皂草素的IGRP206-214dtSCT四聚体在体外能够靶向清除NOD鼠抗原特异性CTL。更多还原

【Abstract】 On the basis of InsB15-23 dtSCT prokaryotic expression vector,dtSCT type of IGRP206-214H-2Kd prokaryotic expression vector was construct.The obtained fusion protein IGRP206-214 H-2Kd monomer was biotinylated,then the IGRP206-214 dtSCT tetramer was coupled with saporin.The spleen lymphocytes from NOD were co-cultured with saporinCoupled dtSCT IGRP206-214H-2Kd tetramer and the IGRP206-214-specific CTLs were detected by Flow Cytometry.Co-incubated NOD spleen lymphocytes together with Saporin Coupled dtSCT IGRP206-214H-2Kdtetramer,the IGRP206-214-specific CTLs significantly decreased compared to the PBS control group(P<0.001,n=6).These results showed that Saporin Coupled dtSCT IGRP206-214H-2Kd tetramer can selectively clear NOD specific CTLs in vitro.更多还原