【摘要】 目的对比研究聚桂醇、无水乙醇及生理盐水经超声介入硬化治疗荷瘤肝癌模型的作用效果,探讨聚桂醇能否成为治疗原发性肝癌的新型硬化剂。方法取BALB/C裸鼠30只及人肝癌Hep G2型细胞系构建裸鼠肝癌模型,将18只造模成功荷瘤裸鼠随机分为聚桂醇组(n=6)、无水乙醇组(n=6)及生理盐水组(n=6),分别向模型瘤体中央部位注入相应药物和生理盐水进行治疗实验,观察原发性肝癌状况;超声测量各组瘤体大小,计算肿瘤生长抑制率,苏木精-伊红染色观察瘤体超声影像学改变,镜下观察肿瘤内部及周围病理学改变。结果实验鼠造模成功18只,皮下瘤体结节均肉眼可见。聚桂醇组和无水乙醇组肿瘤生长明显受抑制(瘤体缩小,1例完全消失),总有效率100%,肿瘤生长抑制率达77.13%,病理学检查见肿瘤组织大片坏死,细胞核固缩;生理盐水组瘤体体积随时间增加无缓解。结论聚桂醇可抑制BALB/C裸鼠原发性肝癌模型,达到与无水乙醇相似的近期硬化效果,有望成为治疗肝癌的理想硬化剂。更多还原

【Abstract】 Objective To compare the therapeutic effects of different hardening agents,including lauromacrogol,ethanol and saline,in treating nude mouse models bearing liver cancer with interventional sclerotherapy,and to discuss the possibility of using lauromacrogol as a new type of hardening agent in the treatment of primary liver cancer. Methods Human hepatocellular carcinoma cell line Hep G2 and 30 BALB / C nude mice were used to establish the liver cancer model. The eighteen successfully established tumor-bearing models were randomly divided into lauromacrogol group( n = 6),ethanol group( n = 6) and saline group( n= 6). Guided by ultrasound,lauromacrogol,ethanol and saline were respectively injected into the central part of the tumor in the experimental mice of the corresponding group; the changes of the liver tumor were recorded. The tumor size was measured by ultrasound and the tumor growth inhibitory rate was calculated.Hematoxylin and eosin staining was used to display the pathological changes of live cancer after treatment.Microscope was used to observe the pathological changes inside and around the tumor. Results The tumorbearing model was successfully established in 18 nude mice,and the subcutaneous tumor nodules were visible to the naked eye. The tumor growth was significantly inhibited in both lauromacrogol group and ethanol group,which presented as a reduction in tumor volume or even complete disappearance of tumor( n = 1); the total effective rate was 100% and the inhibition rate of tumor growth was up to 77. 13%. Pathologically,the tumor tissue displayed larger necrosis and nuclear condensation. In saline group the tumor volume showed no reduction with time. Conclusion Lauromacrogol can inhibit tumor growth in BALB / C nude mice bearing liver cancer,its short-term hardening effect is similar to that of ethanol. Therefore,it is expected that lauromacrogol may become an ideal hardening agent for the treatment of liver cancer.更多还原