【摘要】 目的:探讨穿梭箱训练对大鼠海马缺血后糖原合成酶激酶-3β(GSK-3β)表达及对神经干细胞增殖分化的影响机制。方法:采用大脑中动脉栓塞(MCAO)制作大鼠血管性痴呆模型,免疫组织化学检测穿梭箱训练后海马神经干细胞BrdU的表达,观察穿梭箱训练对血管性痴呆大鼠神经干细胞增殖分化的影响。检测缺血海马GSK-3β的表达,观察穿梭箱训练对血管性痴呆大鼠GSK-3β的影响。结果:脑缺血再灌注第3天缺血海马神经元BrdU阳性细胞明显增多,第7天达高峰。GSK-3β反应产物脑缺血再灌注第7天较正常组增多,随缺血再灌注时间的进一步延长逐渐减少,第14天后持续低水平表达。穿梭箱训练后BrdU、GSK-3β蛋白的表达明显增加。结论:穿梭箱训练促进神经干细胞的增殖及缺血脑组织GSK-3β的表达,其作用可能由GSK-3β信号介导。更多还原

【Abstract】 Objective:To study the effect of behavior training on the expression of GSK-3β and hippocampal neurogenesis in a rat model of cerebral ischemia/reperfusion,and to explore the related molecular and behavior training mechanism.Methods:Behavior training was performed at day 1 after the ischemia/reperfusion.Middle cerebral artery occlusion(MCAO) was performed to establish the vascular dementia model.The expression of BrdU and GSK-3β in the hippocampus was detected with immunohistochemical methods to explore the related neurogenesis and behavior training molecular mechanism Results:BrdU positive cells in the hippocampus obviously increased at day 3 and peaked at day 7 of ischemia/reperfusion.GSK-3β reaction products increased gradually with the time of cerebral ischemia/reperfusion,reached the peak on day 7,and gradually decreased.After training,the expression of GSK-3β in hippocampous had apparently increased in each group.Conclusion:Behavior training promotes the expression of GSK-3β in the hippocampus tissues.These findings indicate that promotion of neural stem cell proliferation induced by behavior training may be mediated by GSK-3β signaling pathway.更多还原