【摘要】 目的观察异甘草素对大鼠脑胶质瘤组织单核细胞趋化蛋白-1(MCP-1)和白介素-1(IL-17)表达的影响,探讨异甘草素抗胶质瘤作用机制。方法方法清洁级Wistar雌性大鼠,n=36只,随机分为3组:假手术组(n=12),模型组(n=12)与异甘草素组(n=12)。异甘草素组腹腔注射异甘草素(500μg/kg/d),模型组腹腔注射等量生理盐水,连续给药一周后处死大鼠。免疫组织化学和Western blot方法检测肿瘤组织MCP-1和IL-17蛋白表达水平的变化。应用CCK-8试剂盒检测异甘草素对人U87胶质瘤细胞增殖能力的影响。结果与假手术组相比,模型组与异甘草素组大鼠脑组织MCP-1和IL-17的蛋白表达的平均光密度值显著增加(P<0.01),而异甘草素组显著低于模型组(P<0.01)。与对照组相比,异甘草素显著抑制了人U87胶质瘤细胞增殖。结论异甘草素抑制人U87胶质瘤细胞增殖可能与降低脑胶质瘤大鼠MCP-1和IL-17的表达相关。更多还原

【Abstract】 Objective To investigate the effect of isoliquiritigenin on the expressions of monocyte chemoattractant protein-1(MCP-1) and interlukin-17(IL-17) in C6 glioma rat models and to explore the antiglioma mechanism. Methods 36 Wistar female rats were randomly divided into 3 group: sham group(n=12)and model group(n=12,intraperitoneal injection of normal saline) and isoliquiritigenin group(n=12,intraperitoneal injection of isoliquiritigenin of 500 μg/ kg/d). The expressions of MCP-1 and IL-17 were detected by immunohistochemistry and Western blot, and CCK-8 kit was applied to detect the proliferation of U87 glioma cells. Results The mean optic density(MOD) values of MCP-1 and IL-17 positive expressions in brain tissues were higher significantly in model group than in sham group(P <0.01), but lower in isoliquiritigenin group than in model group( P <0.01). Compared with control group, isoliquiritigenin significantly inhibited the proliferation of U87 cells, Conclusion The inhibition effect of isoliquiritigenin on glioma cells might be related to the downregulation of MCP-1 and IL-17 expression in rats with brain glioma.更多还原