【摘要】 目的:探讨高危型人乳头瘤病毒(HR-HPV)感染与宫颈鳞癌组织中γ-干扰素(IFN-γ)及白介素-10(IL-10)表达的相关性.方法:选取宫颈上皮内瘤变(CIN)及宫颈鳞癌组织共81例作为研究组,另选取15例正常宫颈组织作为对照组.采用导流杂交基因芯片技术检测宫颈脱落细胞中HR-HPV的感染状态及分型;应用免疫组化方法检测宫颈组织标本中IFN-γ及IL-10的表达并采用图象分析技术对其阳性表达进行半定量分析.结果:(1)正常宫颈组、CIN组和宫颈鳞癌各组的HR-HPV感染率比较有显著差异(P<0.05).(2)正常宫颈组、CIN组及宫颈鳞癌组中IFN-γ阳性表达的IOD值分别为(3.556±1.356)、(3.085±1.081)及(1.617±0.754),经方差分析,组间比较有显著差异(P<0.05);IL-10阳性表达的IOD值分别为(3.384±1.310)、(4.381±1.295)及(7.276±1.985),组间比较差异显著(P<0.05).(3)在HR-HPV非感染组与感染组中IFN-γ阳性表达的IOD值分别为(3.340±1.053)和(2.481±1.210),IL-10阳性表达的IOD值分别为(3.590±1.350)和(5.772±2.048),两组比较均有统计学差异(P<0.05).结论:宫颈局部IFN-γ的表达减弱,IL-10的表达增强,与HR-HPV的感染结局相关.更多还原

【Abstract】 Aim: To study the relationship between the expression of IFN-γ,IL-10 in the cervical lesions and the infection of high-risk human papillomavirus. Methods: 81 cervical tissues with pathologically were confirmed to be cervical intraepithelial neoplasia or cervical squamous cell carcinoma. Another15 cases from normal subjects served as controls. All specimens from the cervical canal were gathered for HPV-DNA and the genetic type of HPV-DNA detection by using flow-through hybridization and gene chip( Hybri Max) through formalin and paraffin processing,and were detected for IFN-γ and IL-10 expression by using streptavidin-peroxidase of immunohistochemistry of integrate optic density( IOD). Results:( 1)The HR-HPV infection rate of CINs and cervical squamous cell carcinoma was significant different from the control group( P < 0. 05).( 2) The IOD of IFN-γ positive expression in the controls,CINs and cervi-cal squamous cell carcinoma were( 3. 556 ± 1. 356),( 3. 085 ± 1. 081),and( 1. 617 ± 0. 754). The expression of IFN-γ in cervical squamous cell carcinoma was lower than that in CINs and the control group( P < 0. 05). IL-10( IOD) positive expression over the controls,CINs,and cervical squamous cell carcinoma were founded to be( 3. 384 ± 1. 310),( 4. 381 ± 1. 295),( 7. 276 ± 1. 985)( P < 0. 05).( 3) The IOD of IFN-γ positive expression in the HR-HPV-positive group and HR-HPV-negative group were( 3. 340 ± 1. 053),( 2. 481 ± 1. 210)( P < 0. 05). Meanwhile,the IOD of IL-10 positive expression in the HR-HPV-positive expression was lower( 3. 590 ± 1. 350) than that of HR-HPV-negative group( 5.772 ± 2. 048). Conclusion: That the expression of IFN-γ decreased of IL-10 enhanced over local cervical tissues will indicate the lasting infection of HPV.更多还原