【摘要】 前期降解试验表明2株能够高效降解阿特拉津的菌株:伯克霍尔德氏菌（Burkholderia sp.）LH-ZY01和丛毛单胞菌（Comamonas sp.）A2混合后降解效果明显提高,因此共降解具有重要的应用价值。为节约生产成本,通过响应面设计,利用2株能够高效降解阿特拉津的菌株,通过一系列显著因素优化设计,最终获得高密度培养上述2株菌的发酵培养基配方（g/L）:蔗糖6.72,葡萄糖6.75,豆粕粉6.34,K₂HPO₄·3H₂O 0.6,KH₂PO₄2.5,MgSO₄·7H₂O 0.3,NaCl 0.4,酵母粉3,pH 7.0⁷.2。通过优化发酵配方和发酵方法,获得发酵液的总菌数为102亿/m L,其中伯克霍尔德氏菌菌数为62亿/m L,丛毛单胞菌菌数为40亿/m L。高密度发酵的优化配方为高效降解阿特拉津复合微生物的生产应用提供了重要依据。更多还原

【Abstract】 In the previous period,atrazine degrading efficiency was improved obviously by using the mixture of two atrazine-degrading bacteria of Burkholderia sp. LH- ZY01 and Comamonas sp. A2.Therefore,their co-degradation has important application value. In order to save the cost of production,statistical approaches were employed for the optimization of the co-culture medium of these two strains. By using Plackett-Burman,steepest ascent and central composite designs,the optimal medium for co-cultivation was obtained as follows（ g / L） : sucrose 6. 72,glucose 6. 75,soybean 6. 34,and yeast powder 3,K₂HPO₄· 3H₂O 0. 6,KH₂PO₄2. 5,MgSO₄· 7H₂O 0. 3,NaCl 0. 4,pH 7. 0-7. 2. After cultivation,the CFU under the optimal medium was 1. 02 × 1011/ m L,in which Burkholderia sp. LH-ZY01 was 6. 2×1010/ m L and Comamonas sp. A2 was 4. 0×1010/ m L. The optimized medium for high density fermentation provides important basis for the application of these strains.更多还原