【摘要】 取人参叶片作为外植体诱导愈伤组织,利用二元二次通用旋转组合设计数学模型筛选植物激素浓度组合,结果表明:2,4-D与KT对人参叶片外植体诱导愈伤组织方程为:Y=0.653 30+0.229 63X1+0.140 85X2-0.130 83X12-0.197 48X22+0.100 00X1X2;叶片诱导愈伤组织最佳培养基为MS附加7 mg·L-12,4-D和0.9 mg·L-1KT,诱导率最高可达80%以上。更多还原

【Abstract】 The plant hormone concentration combinations was selected with the mathematical model designed by dyadic and square general rotary combination by obtaining the Panax ginseng lamina as explant to induce the callus. The result showed that the equation of 2,4- D and KT of Panax ginseng lamina callus induction was Y = 0. 653 30 + 0. 229 63X1+0. 140 85X2- 0. 130 83X12- 0. 197 48X22+ 0. 100 00X1X2. The optimum medium of lamina induction callus was MS added to 7 mg·L- 12,4- D and 0. 9 mg·L- 1KT. The maximum inductivity was more than 80%.更多还原